用于检测食品和饮料中日落黄的量子点连接免疫吸附测定法（QLISA）和酶联免疫吸附测定法（ELISA）的开发。
Development of quantum dot-linked immunosorbent assay (QLISA) and ELISA for the detection of sunset yellow in foods and beverages.
作者信息
Xu Long, Yang Fanfan, Dias Alberto C P, Zhang Xiaoying
机构信息
College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China; Centre of Molecular and Environmental Biology, University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal; College of Pharmacy and Chemistry & Chemical Engineering, Taizhou University, 93 Ji Chuan Road, Taizhou 225300, China.
Centre of Molecular and Environmental Biology, University of Minho, Department of Biology, Campus de Gualtar, 4710-057 Braga, Portugal.
出版信息
Food Chem. 2022 Aug 15;385:132648. doi: 10.1016/j.foodchem.2022.132648. Epub 2022 Mar 7.
Sunset yellow (SY) is widely used as food colorant. Excess and illegal use of SY could pose potential health risk. Quantum dots have been successfully used in biological research due to the high photoluminescence and high resistance to photobleaching. To analyze SY efficiently, quantum dot-linked immunosorbent assay (QLISA) and enzyme-linked immunosorbent assay (ic-ELISA) were developed on the basis of generated monoclonal antibody. A carboxyl group was introduced to SY and coupled with carrier proteins to synthesize artificial antigen. Under the optimal conditions, inhibitory concentrations (IC) of SY were 1.9 ng/mL (ic-ELISA) and 3.4 ng/mL (QLISA); the limits of detection (LODs) were 0.2 ng/mL (ic-ELISA) and 1.0 ng/mL (QLISA), respectively. Cross-reactivities of the mAb toward eight kinds of analogues were<0.01%. The recovery rates in spiked foods and beverages were 75.6%∼120.1% (ic-ELISA) and 74.0%∼114.1% (QLISA), respectively.
日落黄（SY）被广泛用作食品着色剂。过量和非法使用SY可能会带来潜在的健康风险。量子点由于具有高光致发光性和高抗光漂白性，已成功应用于生物学研究。为了高效分析SY，基于产生的单克隆抗体开发了量子点连接免疫吸附测定法（QLISA）和酶联免疫吸附测定法（ic-ELISA）。将羧基引入SY并与载体蛋白偶联以合成人工抗原。在最佳条件下，SY的抑制浓度（IC）分别为1.9 ng/mL（ic-ELISA）和3.4 ng/mL（QLISA）；检测限（LOD）分别为0.2 ng/mL（ic-ELISA）和1.0 ng/mL（QLISA）。单克隆抗体对八种类似物的交叉反应率均<0.01%。加标食品和饮料中的回收率分别为75.6%∼120.1%（ic-ELISA）和74.0%∼114.1%（QLISA）。
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