State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Jiangxi Institute of Veterinary Drug and Feedstuff Control, Nanchang, China.
J Hazard Mater. 2022 Jun 5;431:128498. doi: 10.1016/j.jhazmat.2022.128498. Epub 2022 Feb 16.
To prevent the toxic effect of amantadine (AMD) on humans, a sensitive and direct detection method is required. The conventional enzyme-linked immunosorbent assay (ELISA) usually shows a monochromatic gradient color variation with the concentration of the target; hence, it is not a sensitive method for naked-eye detection. In this work, Ag-Au nanorings with highly tunable plasmon properties were synthesized as colorimetric nanosensors. The growth of Ag on the hollow nanorings led to rich color variations. Ag-Au nanorings were integrated into ELISA for the sensitive detection of AMD with the naked eye. The proposed method showed high sensitivity for the qualitative and quantitative detection of AMD, the visible cut-off value (0.2 ng mL) and limit of detection (0.071 ng mL) were 10-fold and 4.7-fold lower, respectively, than those of conventional ELISA. This method showed a linear range of 0.08-2 ng mL and 4-12 ng mL. The detection results of this method on 100 samples (food samples and municipal water) agreed well with those of liquid chromatography-tandem mass spectrometry. The proposed plasmonic ELISA has high sensitivity, easy operation, and naked-eye readout.
为了防止金刚烷胺（AMD）对人体的毒性作用，需要一种灵敏且直接的检测方法。传统的酶联免疫吸附测定（ELISA）通常会随着目标物浓度的变化呈现出单色梯度颜色变化，因此，它不是一种用于肉眼检测的灵敏方法。在这项工作中，合成了具有高度可调等离子体特性的 Ag-Au 纳米环作为比色纳米传感器。Ag 在空心纳米环上的生长导致了丰富的颜色变化。Ag-Au 纳米环被整合到 ELISA 中，用于 AMD 的肉眼灵敏检测。所提出的方法对 AMD 的定性和定量检测表现出很高的灵敏度，可见截止值（0.2ng/mL）和检测限（0.071ng/mL）分别比传统 ELISA 低 10 倍和 4.7 倍。该方法的线性范围为 0.08-2ng/mL 和 4-12ng/mL。该方法对 100 个样品（食品样品和市政用水）的检测结果与液相色谱-串联质谱法的检测结果吻合良好。所提出的等离子体 ELISA 具有灵敏度高、操作简单和肉眼可读的优点。
