State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China; Institute of Nanochemistry and Nanobiology, School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, China.
Anal Chim Acta. 2022 May 29;1209:339838. doi: 10.1016/j.aca.2022.339838. Epub 2022 Apr 19.
Herein, a facile and sensitive dual mode sensing strategy for amantadine (AMD) level evaluation by coupling the plasmonic Au nanorod (NR) and supramolecular SH-cyclodextrin (CD) through strong Au-S bond is proposed. Methylene blue (MB) molecules can be inserted into the cavity of CD molecules through the hydrophobic interaction, which would cause the plasmon resonance energy transfer (PRET) process as well as electrochemical signal response due to the spectrum overlap between Au NR and MB molecules and the electrochemical conversion activity of MB molecules. Subsequently, AMD would induce the replacement of MB molecules because of the stronger interaction with CD, resulting the recovery of scattering intensity of Au NR and decrease of the electrooxidation current of MB. On one hand, the increase of Au NR scattering intensity is linearly proportional to AMD with the concentration from 0.4 to 3.0 μM, and reaches a limit of detection (LOD) of 0.28 μM. On the other hand, electrochemical measurement method enlarged the detection range of AMD. The variation of electrochemical oxidation peak current of MB is linearly proportional to the logarithm value of AMD concentration from 2.5 to 375.0 μM, with LOD of 1.9 μM. Subsequently, the proposed dual mode response sensing strategy was successfully employed for the detection of AMD in human serum samples with great selectivity and sensitivity, with a recovery percentage ranged from 92.6 to 112.0%. Overall, this Au NR@SH-CD-MB nanoparticle based single particle plasmonic and electrochemical dual mode sensing method provides great potential in the field of clinical drug detection or metabolic process investigation in the future.
本文提出了一种通过强 Au-S 键将等离子体 Au 纳米棒 (NR) 和超分子 SH-环糊精 (CD) 耦合起来的简便、灵敏的金刚烷胺 (AMD) 水平评估双模式传感策略。亚甲蓝 (MB) 分子可以通过疏水相互作用插入到 CD 分子的空腔中，由于 Au NR 和 MB 分子之间的光谱重叠以及 MB 分子的电化学转换活性，会导致等离子体共振能量转移 (PRET) 过程和电化学信号响应。随后，由于与 CD 的更强相互作用，AMD 会诱导 MB 分子的取代，从而导致 Au NR 散射强度的恢复和 MB 分子的电氧化电流的降低。一方面，Au NR 散射强度的增加与 AMD 呈线性比例关系，浓度范围为 0.4 至 3.0 μM，检测限 (LOD) 为 0.28 μM。另一方面，电化学测量方法扩大了 AMD 的检测范围。MB 的电化学氧化峰电流的变化与 AMD 浓度的对数呈线性比例关系，浓度范围为 2.5 至 375.0 μM，检测限为 1.9 μM。随后，该双模式响应传感策略成功应用于人血清样品中 AMD 的检测，具有良好的选择性和灵敏度，回收率百分比在 92.6%至 112.0%之间。总体而言，这种基于 Au NR@SH-CD-MB 纳米粒子的单颗粒等离子体和电化学双模式传感方法在未来的临床药物检测或代谢过程研究领域具有很大的潜力。
