College of Plant Protection Shandong Agricultural University, Tai'an, Shandong 271018, PR China; College of Resources and Environment Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
College of Plant Protection Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
J Hazard Mater. 2022 Jun 5;431:128626. doi: 10.1016/j.jhazmat.2022.128626. Epub 2022 Mar 4.
Thifluzamide, a succinate dehydrogenase inhibitor fungicide, has been used extensively for many diseases control and has the risk of accumulation in soil ecology. In order to study the ecotoxicity of thifluzamide to soil fungal communities, typical corn field soils in north (Tai'an) and south (Guoyang) China were treated with thifluzamide (0, 0.1, 1.0 and 10.0 mg/kg) and incubated for 60 days. Thifluzamide exposure promoted soil basal respiration, and significantly reduced the number of soil culturable fungi and the abundance of soil fungi (RT-qPCR) in middle and late treatment period (15, 30, 60 days). Illumina Mi-Seq sequencing revealed that thifluzamide could reduce fungal alpha diversity (Sobs, Shannon, Simpson indexes) and change fungal community structure. FUN Guild analysis showed that the relative abundance of Undefined Saprotroph increased after the thifluzamide treatment, whereas that of Plant Pathogen decreased, and we concluded that exposure to thifluzamide could change the function of soil fungi. This study evaluated the soil ecological risk caused by thifluzamide's release into soil, providing a basis for its rational application.
噻呋酰胺是一种琥珀酸脱氢酶抑制剂类杀菌剂，被广泛用于多种病害的防治，存在在土壤生态系统中积累的风险。为研究噻呋酰胺对土壤真菌群落的生态毒性，本研究采用室内培养试验，以中国北方（泰安）和南方（固阳）典型玉米田土壤为供试土壤，设置 0、0.1、1.0 和 10.0 mg/kg 4 个噻呋酰胺处理，培养 60 d。结果表明，噻呋酰胺处理显著促进了土壤基础呼吸，且在中、后期（15、30、60 d）显著降低了土壤可培养真菌数量和真菌丰度（RT-qPCR）。Illumina Mi-Seq 高通量测序结果显示，噻呋酰胺降低了土壤真菌 α 多样性（Sobs、Shannon、Simpson 指数）并改变了真菌群落结构。FUN Guild 分析表明，噻呋酰胺处理后土壤中未明腐生真菌的相对丰度增加，而植物病原菌的相对丰度降低，说明噻呋酰胺暴露可能改变了土壤真菌的功能。本研究评估了噻呋酰胺释放到土壤中对土壤生态系统造成的风险，为其合理应用提供了依据。
