Yang Xiangxia, Yuan Rongwei, Yang Shuangyu, Dai Zhian, Di Na, Yang Haijun, He Zhili, Wei Mi
School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-Sen University, Shenzhen, 518107, China.
Center for Basic Experiment and Practice Training, South China Agricultural University, Guangzhou, 510462, China.
Microbiome. 2024 Dec 20;12(1):270. doi: 10.1186/s40168-024-01969-9.
Mangrove plants growing in the high salt environment of coastal intertidal zones colonize a variety of microorganisms in the phyllosphere, which have potential salt-tolerant and growth-promoting effects. However, the characteristics of microbial communities in the phyllosphere of mangrove species with and without salt glands and the differences between them remain unknown, and the exploration and the agricultural utilization of functional microbial resources from the leaves of mangrove plants are insufficient.
In this study, we examined six typical mangrove species to unravel the differences in the diversity and structure of phyllosphere microbial communities between mangrove species with or without salt glands. Our results showed that a combination of salt-tolerant growth-promoting strains of Pantoea stewartii A and Bacillus marisflavi Y25 (A + Y25) was constructed from the phyllosphere of mangrove plants, which demonstrated an ability to modulate osmotic substances in rice and regulate the expression of salt-resistance-associated genes. Further metagenomic analysis revealed that exogenous inoculation with A + Y25 increased the rice rhizosphere's specific microbial taxon Chloroflexi, thereby elevating microbial community quorum sensing and ultimately enhancing ionic balance and overall microbial community function to aid salt resistance in rice.
This study advances our understanding of the mutualistic and symbiotic relationships between mangrove species and their phyllosphere microbial communities. It offers a paradigm for exploring agricultural beneficial microbial resources from mangrove leaves and providing the potential for applying the salt-tolerant bacterial consortium to enhance crop adaptability in saline-alkaline land. Video Abstract.
生长在沿海潮间带高盐环境中的红树林植物在叶际定殖了多种微生物，这些微生物具有潜在的耐盐和促生长作用。然而，有无盐腺的红树林物种叶际微生物群落特征及其差异尚不清楚，红树林植物叶片功能性微生物资源的开发与农业利用也不足。
在本研究中，我们检测了六种典型的红树林物种，以揭示有无盐腺的红树林物种叶际微生物群落多样性和结构的差异。我们的结果表明，从红树林植物叶际构建了耐盐促生长菌株斯氏泛菌A和海黄芽孢杆菌Y25（A+Y25）的组合，该组合具有调节水稻渗透物质和调控耐盐相关基因表达的能力。进一步的宏基因组分析表明，外源接种A+Y25增加了水稻根际特定微生物分类群绿弯菌门，从而提高了微生物群落的群体感应，最终增强了离子平衡和整体微生物群落功能，以帮助水稻耐盐。
本研究增进了我们对红树林物种与其叶际微生物群落之间互利共生关系的理解。它为从红树林叶片中探索农业有益微生物资源提供了范例，并为应用耐盐细菌联合体提高作物在盐碱地的适应性提供了潜力。视频摘要。
