Liu Yanfei, Huang Hui, Li Yiwen, Cui Jing, Tong Tiejun, Yang Hongjun, Liu Yue
National Clinical Research Centre for Chinese Medicine Cardiology, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
Second Department of Geriatrics, Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing, China.
Front Med (Lausanne). 2022 Feb 24;9:737662. doi: 10.3389/fmed.2022.737662. eCollection 2022.
A quantitative method for the evaluation of facial swelling in rats with middle cerebral artery occlusion (MCAO) was established using a mathematical method for the first time. The rat model of MCAO was established bilateral common carotid artery ligation. Three groups of rats with the same baseline were selected (model group, positive drug group, and control group) according to their behavioral score and body weight 24 h after surgery. Drug administration was initiated on post-MCAO day 8 and was continued for 28 days. Mobile phones were used to collect facial images at different time points after surgery. In facial image analysis, the outer canthi of both eyes were used as the facial dividing line, and the outer edge of the rat's face was framed using the marking method, and the framed part was regarded as the facial area (S) of the rats. The histogram created with Photoshop CS5 was used to measure the face area in pixels. The distance between the outer canthi of both eyes (Le) and vertical line from the tip of the nose to the line joining the eyes was recorded as H1, and the line from the tip of the nose to the midpoint of the line joining the eyes was recorded as H2. The facial area was calibrated based on the relationship between H1 and H2. The distance between the eyes was inversely proportional to the distance between the rats and mobile phone such that the face area was calibrated by unifying Le. The size of Le between the eyes was inversely proportional to the distance between the rats and mobile phone. This was used to calibrate the face area. When compared with the control group, the facial area of the model group gradually increased from postoperative day 1 to day 7, and there was a significant difference in the facial area of the model group on postoperative day 7. Hence, positive drugs exhibited the effect of improving facial swelling. H1 and H2 can reflect the state of turning the head and raising the head of the rats, respectively. Facial area was calibrated according to the relationship between H1 and H2, which had no obvious effect on the overall conclusion. Furthermore, mobile phone lens was used to capture the picture of rat face, and the distance between the eyes and H1 and H2 was used to calibrate the facial area. Hence, this method is convenient and can be used to evaluate subjective judgment of the human eyes a quantitative method.
首次采用数学方法建立了一种定量评估大脑中动脉闭塞（MCAO）大鼠面部肿胀的方法。通过双侧颈总动脉结扎建立MCAO大鼠模型。根据术后24小时的行为评分和体重，选择三组基线相同的大鼠（模型组、阳性药物组和对照组）。在MCAO术后第8天开始给药，并持续28天。术后不同时间点使用手机采集面部图像。在面部图像分析中，以双眼外眦作为面部分界线，采用标记法勾勒出大鼠面部的外缘，将勾勒出的部分视为大鼠的面部面积（S）。使用Photoshop CS5创建的直方图来测量以像素为单位的面部面积。记录双眼外眦之间的距离（Le）以及从鼻尖到连接双眼的线的垂直线段为H1，从鼻尖到连接双眼的线的中点的线段为H2。根据H1和H2之间的关系对面部面积进行校准。双眼之间的距离与大鼠和手机之间的距离成反比，从而通过统一Le来校准面部面积。双眼之间Le的大小与大鼠和手机之间的距离成反比，以此来校准面部面积。与对照组相比，模型组的面部面积从术后第1天到第7天逐渐增加，术后第7天模型组的面部面积有显著差异。因此，阳性药物具有改善面部肿胀的作用。H1和H2分别可以反映大鼠转头和抬头的状态。根据H1和H2之间的关系对面部面积进行校准，对总体结论没有明显影响。此外，使用手机镜头拍摄大鼠面部图片，并利用双眼距离以及H1和H2来校准面部面积。因此，该方法简便易行，可用于替代人眼主观判断的定量方法。
