Institut National de la Santé et de la Recherche Médicale, Centre de Recherche des Cordeliers, Sorbonne Université, Université de Paris, Paris, France.
Functional Genetics of Infectious Diseases Unit, Department of Global Health, Institut Pasteur, Paris, France.
Front Immunol. 2022 Feb 24;13:838448. doi: 10.3389/fimmu.2022.838448. eCollection 2022.
Basophils play a key role in the orientation of immune responses. Though the interaction of SARS-CoV-2 with various immune cells has been relatively well studied, the response of basophils to this pandemic virus is not characterized yet. In this study, we report that SARS-CoV-2 induces cytokine responses and in particular IL-13, in both resting and IL-3 primed basophils. The response was prominent under IL-3 primed condition. However, either SARS-CoV-2 or SARS-CoV-2-infected epithelial cells did not alter the expression of surface markers associated with the activation of basophils, such as CD69, CD13 and/or degranulation marker CD107a. We also validate that human basophils are not permissive to SARS-CoV-2 replication. Though increased expression of immune checkpoint molecule PD-L1 has been reported on the basophils from COVID-19 patients, we observed that SARS-CoV-2 does not induce PD-L1 on the basophils. Our data suggest that basophil cytokine responses to SARS-CoV-2 might help in reducing the inflammation and also to promote antibody responses to the virus.
嗜碱性粒细胞在免疫反应的定向中发挥关键作用。尽管 SARS-CoV-2 与各种免疫细胞的相互作用已经得到了相对较好的研究，但嗜碱性粒细胞对这种大流行病毒的反应尚未得到明确描述。在这项研究中，我们报告 SARS-CoV-2 诱导静止和 IL-3 预刺激的嗜碱性粒细胞产生细胞因子反应，特别是 IL-13。在 IL-3 预刺激条件下，反应更为明显。然而，SARS-CoV-2 或 SARS-CoV-2 感染的上皮细胞并没有改变与嗜碱性粒细胞激活相关的表面标志物的表达，例如 CD69、CD13 和/或脱颗粒标志物 CD107a。我们还验证了人类嗜碱性粒细胞对 SARS-CoV-2 复制不具有易感性。尽管 COVID-19 患者的嗜碱性粒细胞上报告了免疫检查点分子 PD-L1 的表达增加，但我们观察到 SARS-CoV-2 不会诱导嗜碱性粒细胞上的 PD-L1。我们的数据表明，SARS-CoV-2 引起的嗜碱性粒细胞细胞因子反应可能有助于减轻炎症，并促进针对该病毒的抗体反应。
