1Bryansk City Hospital No. 1, Bryansk, Russian Federation; 2Voronezh State Medical University N. N. Burdenko, Voronezh, Russian Federation; 3Bryansk State Technical University, Bryansk, Russian Federation; 4Penza State University, Penza, Russian Federation.
Acta Clin Croat. 2022 Feb;60(3):467-475. doi: 10.20471/acc.2021.60.03.18.
The aim of the study was to investigate morphological traits of hepatic parenchymal tissue repair in response to injury using the conventional technique (closure) and an innovation method (such as hemostatic medication swab packing and modified batching). The experimental study was carried out on laboratory rats of the Winzar breed using light microscopy, standard stains for micropreparations, and morphometry. Histopathologic examination of micropreparations stained by standard methods revealed pronounced dystrophic processes in hepatocytes located near the necrotic zone (albuminous and hydropic degeneration and chromatin fragmentation in the nuclei). Morphometric studies showed a significant decrease (p<0.001) in almost all indicators of the size of cells and nuclei both near necrosis and distant from it on day 28 of the experiment in the experimental group in comparison to the control group. The results obtained pointed to more intense repair processes when applying the innovation method.
本研究旨在通过常规技术（闭合）和创新方法（如止血药物拭子填塞和改良分批），研究肝实质组织损伤后的形态学修复特征。采用光镜、微制备标准染色和形态计量学方法，对温扎尔品种实验大鼠进行了实验研究。用标准方法染色的微制备的组织病理学检查显示，靠近坏死区的肝细胞（白蛋白和水肿变性以及核内染色质片段化）存在明显的营养不良过程。形态计量学研究表明，与对照组相比，实验组在实验第 28 天，靠近和远离坏死区的几乎所有细胞和核大小指标均显著降低（p<0.001）。研究结果表明，采用创新方法可促进更强烈的修复过程。
