Zehner M M, Farnsworth R J, Appleman R D, Larntz K, Springer J A
J Dairy Sci. 1986 Jul;69(7):1932-41. doi: 10.3168/jds.S0022-0302(86)80620-8.
The objective of the study was to determine whether, under controlled conditions, bedding materials vary in their ability to support growth of different environmental pathogens independent of the presence of feces, urine, or other contamination. Five sterilized bedding materials (fine hardwood chips, recycled dried manure, chopped newspaper, softwood sawdust, and chopped straw) and three bacterial species (Escherichia coli, Klebsiella pneumoniae, and Streptococcus uberis) were used for a total of 15 bedding/bacteria combinations, replicated in three trials. Samples were incubated at 37 degrees C, and bacterial counts were determined over 5 d. Rapid growth was seen in straw and recycled manure, some growth occurred in hardwood chips, and a rapid decline in bacterial counts was observed in paper and softwood sawdust. In general, K. pneumoniae and E. coli showed more rapid growth or less rapid decline than did S. uberis. These results demonstrate that clean, damp bedding may support bacterial growth and suggest that high bacterial counts under barn conditions are influenced by factors more complex than type of bedding used.
本研究的目的是确定在受控条件下，垫料在支持不同环境病原体生长方面的能力是否会因粪便、尿液或其他污染物的存在而有所不同。使用了五种经过消毒的垫料（细硬木屑、回收干粪、碎报纸、软木锯末和碎稻草）和三种细菌（大肠杆菌、肺炎克雷伯菌和乳房链球菌），共形成15种垫料/细菌组合，并在三项试验中进行重复。样本在37摄氏度下孵育，并在5天内测定细菌数量。在稻草和回收粪便中观察到细菌快速生长，硬木屑中有一些生长，而在纸张和软木锯末中观察到细菌数量迅速下降。总体而言，肺炎克雷伯菌和大肠杆菌的生长速度比乳房链球菌更快或下降速度更慢。这些结果表明，干净、潮湿的垫料可能支持细菌生长，并表明畜舍条件下的高细菌数量受比所用垫料类型更复杂的因素影响。
