Genecology Research Centre, University of the Sunshine Coast, Maroochydore DC, Queensland, Australia.
School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore DC, Queensland, Australia.
Biofouling. 2022 Feb;38(2):186-206. doi: 10.1080/08927014.2022.2049256. Epub 2022 Mar 14.
are most well known for their production of high-quality natural pearls. They also generate another natural material, the byssus, an adhesive thread critical for steadfast attachment underwater. Herein, byssal threads were analysed proteotranscriptomics to reveal 49 proteins. Further characterisation was undertaken on five highly expressed genes: glycine-rich thread protein (GRT; also known as PUF3), apfp1/perlucin-like protein (Pmfp1); peroxidase; thrombospondin 1, and Balbiani ring 3 (BR3), which showed localised tissue expression. The spatial distribution of GRT and Pmfp1 immunodetection combined with histology helped to identify glandular regions of the foot that contribute to byssal thread production: the byssal gland, the duct gland, and two thread-forming glands of basophilic and acidophilic serous-like cells. This work advanced primary knowledge on the glands involved in the creation of byssal threads and the protein composition of the byssus for , providing a platform for the design of marine biopolymers.
珍珠贝以生产高品质天然珍珠而闻名。它们还产生另一种天然物质——足丝，这是一种水下固定用的粘性线。本文通过蛋白质组学分析了足丝中的 49 种蛋白质。对五个高表达基因进行了进一步的表征：富含甘氨酸的丝蛋白（GRT；也称为 PUF3）、apfp1/类 Perlucin 蛋白（Pmfp1）、过氧化物酶、血栓反应蛋白 1 和 Balbiani 环 3（BR3），它们显示出组织表达的局部化。GRT 和 Pmfp1 的免疫检测与组织学相结合，有助于识别足丝产生的足部腺区：足腺、导管腺和两个由嗜碱性和嗜酸性浆液样细胞组成的丝形成腺。这项工作为了解参与足丝生成的腺体以及足丝的蛋白质组成提供了基础，为海洋生物聚合物的设计提供了一个平台。
