Ministry of Education Key Laboratory of Protein Sciences, School of Life Sciences, Tsinghua University, Beijing, China.
College of Oceanography, Hohai University, Nanjing, China.
Biofouling. 2021 Mar;37(3):299-308. doi: 10.1080/08927014.2021.1901890. Epub 2021 Mar 24.
Byssuses, which are proteinaceous fibers secreted by mollusks, are remarkable underwater adhesives. Although mussel adhesives are well known, much less is known about the byssal proteins of pearl oysters especially in the adhesive regions. In this study, adhesive proteins from the pearl oyster were studied in depth by transcriptomics and proteomics approaches. In total, 16 novel proteins were identified including a von Willebrand factor type A domain-containing protein, a thrombospondin-1-like protein, tyrosinase, mucin-like proteins, protease inhibitors, and unannotated foot protein 3 (PUF3) to PUF6. Interestingly, PUF3-6 are enriched with glycine, serine, and PXG (X = F/Y/W/K/L) motifs and are highly expressed in the foot. The identification of byssal proteins of the pearl oyster is a key step for understanding byssus formation and may inspire the synthesis of novel adhesives for underwater use and the development of anti-biofouling strategies.
贻贝丝是软体动物分泌的蛋白质纤维，是一种出色的水下粘合剂。尽管贻贝粘合剂广为人知，但人们对珍珠贝的贻贝蛋白知之甚少，尤其是在粘合区域。在这项研究中，通过转录组学和蛋白质组学方法深入研究了珍珠贝的粘合蛋白。总共鉴定出 16 种新型蛋白质，包括含有 von Willebrand 因子 A 结构域的蛋白、血栓反应蛋白 1 样蛋白、酪氨酸酶、粘蛋白样蛋白、蛋白酶抑制剂以及未注释的足蛋白 3（PUF3）到 PUF6。有趣的是，PUF3-6 富含甘氨酸、丝氨酸和 PXG（X= F/Y/W/K/L）基序，在足部高度表达。珍珠贝贻贝蛋白的鉴定是理解贻贝形成的关键步骤，可能为水下使用的新型粘合剂的合成以及抗生物污损策略的发展提供启示。
