基于MTT法的使用兰姆提取物对A2780细胞增殖抑制分析及CXCR4和HER2表达情况
MTT assay based inhibition analysis of A2780 cells proliferation using Lam. extract with CXCR4 and HER2 expression.
作者信息
Palanisamy Chella Perumal, Jh Shazia Fathima, Sekar Ramya, Rajagopal Ponnulakshmi, Jeyaraman Selvaraj
机构信息
State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, China.
Department of Oral and Maxillofacial Pathology, Ragas Dental College and Hospitals, Chennai, India.
出版信息
Bioinformation. 2021 Jul 31;17(7):705-709. doi: 10.6026/97320630017705. eCollection 2021.
It is of interest to document the inhibition of A2780 cell proliferation using Lam.() extract by MTT assay and by monitoring the CXCR4 and HER2 expression through RT-PCR analysis. Results shown that the n-hexane extract of have anticancer activity IC values of 32.46±0.92 µg/mL on A2780 cell lines. It is further found that the CXCR4 and HER2 mRNA and protein expression were significantly reduced in treated A2780 cell lines. Thus, the n-hexane extract of is a natural source of bioactive compounds as potential anticancer agents.
通过MTT法以及通过RT-PCR分析监测CXCR4和HER2表达来记录Lam.()提取物对A2780细胞增殖的抑制作用是很有意义的。结果表明，其正己烷提取物对A2780细胞系具有抗癌活性，IC值为32.46±0.92 µg/mL。进一步发现，在经处理的A2780细胞系中，CXCR4和HER2的mRNA及蛋白表达显著降低。因此，其正己烷提取物是作为潜在抗癌剂的生物活性化合物的天然来源。
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