Huang Chunmeng, Wang Zhi, Zhu Pengyu, Wang Chenguang, Wang Chaonan, Xu Wenjie, Li Zhihong, Fu Wei, Zhu Shuifang
College of Plant Protection, China Agricultural University, Beijing, China.
Chinese Academy of Inspection and Quarantine, Beijing, China.
Front Plant Sci. 2022 Feb 24;13:745708. doi: 10.3389/fpls.2022.745708. eCollection 2022.
The use of omics techniques to analyze the differences between genetic engineering organisms and their parents can identify unintended effects and explore whether such unintended effects will have negative consequences. In order to evaluate whether genetic engineering will cause changes in crops beyond the changes introduced by conventional plant breeding, we compared the extent of transcriptome and metabolome modification in the leaves of three lines developed by RNA interference (RNAi)-based genetic engineering and three lines developed by conventional breeding. The results showed that both types of plant breeding methods can manifest changes at the short interfering RNA (siRNA), transcriptomic, and metabolic levels. Relative expression analysis of potential off-target gene revealed that there was no broad gene decline in the three RNAi-based genetic engineering lines. We found that the number of DEGs and DAMs between RNAi-based genetic engineering lines and the parental line was less than that between conventional breeding lines. These unique DEGs and DAMs between RNAi-based genetic engineering lines and the parental lines were not enriched in detrimental metabolic pathways. The results suggest that RNAi-based genetic engineering do not cause unintended effects beyond those found in conventional breeding in maize.
使用组学技术分析基因工程生物与其亲本之间的差异，可以识别非预期效应，并探究此类非预期效应是否会产生负面后果。为了评估基因工程是否会导致作物产生超出传统植物育种所带来的变化，我们比较了基于RNA干扰（RNAi）的基因工程培育的三个品系和传统育种培育的三个品系叶片中转录组和代谢组的修饰程度。结果表明，两种植物育种方法均可在小干扰RNA（siRNA）、转录组和代谢水平上表现出变化。潜在脱靶基因的相对表达分析表明，三个基于RNAi的基因工程品系中没有广泛的基因下调。我们发现，基于RNAi的基因工程品系与亲本之间的差异表达基因（DEG）和差异代谢物（DAM）的数量少于传统育种品系之间的数量。基于RNAi的基因工程品系与亲本之间这些独特的DEG和DAM在有害代谢途径中未富集。结果表明，基于RNAi的基因工程在玉米中不会产生超出传统育种的非预期效应。
