Wu Bin, Li Jia, Peng Dinghua, Wang Ziru, Xu Heng
College of Ecology and Environment, Chengdu University of Technology, Chengdu, China.
State Key Laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu University of Technology, Chengdu, China.
Front Plant Sci. 2022 Feb 25;13:808844. doi: 10.3389/fpls.2022.808844. eCollection 2022.
Vetiver grass ( L.) has been used to remediate cadmium (Cd)-contaminated soil, while there have been few studies on the influence of Cd exposure on the rhizospheric microbial community and transcriptional expression of . In this study, we investigated the response of the rhizospheric microbial community and transcriptional expression of in 20 mg/kg Cd-contaminated soil. The results showed that Cd levels in the roots and shoots of reached 250.80 and 73.40 mg/kg, respectively. The Cd exposure changed the rhizospheric bacterial community, resulting in the significant enrichment of , , and in Cd-contaminated soil. In addition, 880 and 3,419 differentially expressed genes were identified in the plant roots and shoots, respectively, in response to Cd stress. Among these, the overexpressed genes associated with redox homeostasis, glutathione (GSH) metabolism, cell wall biosynthesis, and transmembrane transport pathways were found to participate in Cd detoxification in These findings could be useful for understanding the selective variation of the rhizospheric microbial community and the detoxification mechanisms of in Cd phytoremediation.
香根草（Vetiveria zizanioides (L.)）已被用于修复镉（Cd）污染土壤，然而关于镉暴露对根际微生物群落及Vetiveria zizanioides转录表达的影响的研究却很少。在本研究中，我们调查了香根草在20 mg/kg镉污染土壤中根际微生物群落的响应及转录表达情况。结果表明，香根草根系和地上部分的镉含量分别达到250.80 mg/kg和73.40 mg/kg。镉暴露改变了根际细菌群落，导致镉污染土壤中鞘氨醇单胞菌属（Sphingomonas）、贪铜菌属（Cupriavidus）和芽孢杆菌属（Bacillus）显著富集。此外，分别在植物根系和地上部分鉴定出880个和3419个响应镉胁迫的差异表达基因。其中，发现与氧化还原稳态、谷胱甘肽（GSH）代谢、细胞壁生物合成和跨膜运输途径相关的过表达基因参与了香根草的镉解毒过程。这些发现有助于理解根际微生物群落的选择性变化以及香根草在镉植物修复中的解毒机制。
