Jones P S, Tesser P, Keyser K T, Quitschke W, Samadi R, Karten H J, Schechter N
J Neurochem. 1986 Oct;47(4):1226-34. doi: 10.1111/j.1471-4159.1986.tb00744.x.
The predominant proteins (58K) of the intermediate filament complex in the goldfish visual pathway consist of a series of isoelectric variants. Previous biochemical studies have shown that proteins ON1 and ON2 are of neuronal origin, whereas ON3 and ON4 are of nonneuronal origin. Polyclonal antibodies, purified by affinity chromatography, that are specific for ON1 and ON2 or ON3 and ON4 have been used to localize histologically the ON proteins within the normal and crushed optic nerve. Anti-ON1/ON2 antiserum presented a pattern consistent with intraaxonal staining. A nonneuronal staining pattern was observed with anti-ON3/ON4 antiserum. The two patterns were distinct from and complementary to each other. The data suggest that ON3 and ON4 represent a novel glial fibrillary acidic protein. The results are discussed in terms of the function of these proteins in development, plasticity, and regeneration.
金鱼视觉通路中中间丝复合体的主要蛋白质（58K）由一系列等电变体组成。先前的生化研究表明，蛋白质ON1和ON2起源于神经元，而ON3和ON4起源于非神经元。通过亲和层析纯化的、对ON1和ON2或ON3和ON4具有特异性的多克隆抗体已被用于在组织学上定位正常和挤压视神经内的ON蛋白。抗ON1/ON2抗血清呈现出与轴突内染色一致的模式。用抗ON3/ON4抗血清观察到非神经元染色模式。这两种模式彼此不同且相互补充。数据表明ON3和ON4代表一种新型的胶质纤维酸性蛋白。根据这些蛋白质在发育、可塑性和再生中的功能对结果进行了讨论。
