Marques Manuel J, Hughes Michael R, Uceda Adrián F, Gelikonov Grigory, Bradu Adrian, Podoleanu Adrian
Applied Optics Group, Physics and Astronomy, Division of Natural Sciences, University of Kent, Canterbury CT2 7NH, United Kingdom.
Both authors contributed equally to this publication.
Biomed Opt Express. 2022 Jan 14;13(2):761-776. doi: 10.1364/BOE.444170. eCollection 2022 Feb 1.
Forward-viewing endoscopic optical coherence tomography (OCT) provides 3D imaging , and can be combined with widefield fluorescence imaging by use of a double-clad fiber. However, it is technically challenging to build a high-performance miniaturized 2D scanning system with a large field-of-view. In this paper we demonstrate how a 1D scanning probe, which produces cross-sectional OCT images (B-scans) and 1D fluorescence T-scans, can be transformed into a 2D scanning probe by manual scanning along the second axis. OCT volumes are assembled from the B-scans using speckle decorrelation measurements to estimate the out-of-plane motion along the manual scan direction. Motion within the plane of the B-scans is corrected using image registration by normalized cross correlation. OCT slices and fluorescence images, corrected for probe motion in 3D, can be displayed in real-time during the scan. For a B-scan frame rate of 250 Hz, and an OCT lateral resolution of approximately , the approach can handle out-of-plane motion at speeds of up to 4 mm/s.
前视内镜光学相干断层扫描（OCT）可提供三维成像，并且可以通过使用双包层光纤与宽视野荧光成像相结合。然而，构建一个具有大视野的高性能小型二维扫描系统在技术上具有挑战性。在本文中，我们展示了如何通过沿第二轴手动扫描，将产生横截面OCT图像（B扫描）和一维荧光T扫描的一维扫描探头转变为二维扫描探头。使用散斑去相关测量从B扫描组装OCT体积，以估计沿手动扫描方向的平面外运动。使用归一化互相关的图像配准校正B扫描平面内的运动。经三维探头运动校正的OCT切片和荧光图像可以在扫描期间实时显示。对于250 Hz的B扫描帧率和大约 的OCT横向分辨率，该方法可以处理高达4 mm/s速度的平面外运动。
