Tinti Michele, Ferguson Michael A J
Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, DD1 5HN, UK.
Wellcome Open Res. 2023 Feb 1;7:34. doi: 10.12688/wellcomeopenres.17607.2. eCollection 2022.
is a protozoan parasite and etiological agent of human and animal African trypanosomiasis. It has a complex life cycle, but the most studied cellular types are the cultivated bloodstream- and procyclic-forms. These correspond to the replicating, mammalian host bloodstream-dwelling, slender trypomastigotes and tsetse vector midgut-dwelling procyclic lifecycle stages, respectively. Several proteomics studies have reported the differential abundance of proteins between these cultivated cell types. However, there are no datasets providing protein abundance, from most to least abundant, within and between both cell types. We used MaxQuant software 1.6.10.4 to reprocess a recent large-scale proteomics experiment from our laboratory and extracted intensity-based quantifications of the bloodstream and procyclic form proteomes. We created a web interface to visually explore protein abundances within and between the cultivated bloodstream and procyclic form proteomes. The protein abundance visualization tool, searchable by protein name(s) and attribute(s), is likely to be useful to the trypanosome research community. It will allow users to contextualise their proteins of interest in terms of their abundances in the bloodstream and procyclic form proteomes.
是一种原生动物寄生虫,也是人类和动物非洲锥虫病的病原体。它具有复杂的生命周期,但研究最多的细胞类型是培养的血流型和前循环型。这些分别对应于在哺乳动物宿主血流中复制的细长型锥鞭毛体和采采蝇媒介中肠中的前循环生命周期阶段。几项蛋白质组学研究报告了这些培养细胞类型之间蛋白质丰度的差异。然而,没有数据集提供两种细胞类型内部和之间从最丰富到最不丰富的蛋白质丰度。我们使用MaxQuant软件1.6.10.4对我们实验室最近的一项大规模蛋白质组学实验进行重新处理,并提取了基于强度的血流型和前循环型蛋白质组定量。我们创建了一个网络界面,以直观地探索培养的血流型和前循环型蛋白质组内部和之间的蛋白质丰度。这个可通过蛋白质名称和属性进行搜索的蛋白质丰度可视化工具可能对锥虫研究界有用。它将允许用户根据感兴趣的蛋白质在血流型和前循环型蛋白质组中的丰度来了解其背景情况。