Department of Medical Neurobiology, Institute for Medical Research Israel Canada, Faculty of Medicine, The Hebrew University, Jerusalem 9112001, Israel.
The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, Jerusalem 9112001, Israel.
STAR Protoc. 2022 Mar 4;3(1):101224. doi: 10.1016/j.xpro.2022.101224. eCollection 2022 Mar 18.
This protocol aims to measure ion dynamics in nociceptive terminal endings in intact mice . We describe viral injection of GCaMP6s + RFP into trigeminal ganglia (TG) of mice, followed by calcium imaging of corneal nociceptive terminals that express GCaMP6s and RFP. This fast and high-resolution optical recording technique enables studying a nociceptive terminal's functional molecular network in physiological and pathological conditions. This platform can be applied to studying the physiology of terminals of other neurons. For complete details on the use and execution of this protocol, please refer to Goldstein et al. (2019).
本方案旨在测量完整小鼠伤害性终末内的离子动力学。我们描述了病毒注射到小鼠三叉神经节（TG）中携带 GCaMP6s+RFP 的方法，随后对表达 GCaMP6s 和 RFP 的角膜伤害性终末进行钙成像。这种快速且高分辨率的光学记录技术可用于在生理和病理条件下研究伤害性终末的功能分子网络。该平台可用于研究其他神经元终末的生理学。如需详细了解本方案的使用和执行，请参考 Goldstein 等人（2019 年）的文献。
