Precision Medicine Theme, South Australian Health and Medical Research Institute, Adelaide, SA 5001, Australia.
Flinders Health and Medical Research Institute, Flinders University, Bedford Park, SA 5042, Australia.
STAR Protoc. 2022 Mar 5;3(1):101220. doi: 10.1016/j.xpro.2022.101220. eCollection 2022 Mar 18.
We present this protocol using a mouse model to assess the impact of early-life antibiotic exposure on mammalian lifespan and the composition of the gut microbiota over time. We describe longitudinal fecal sampling and health monitoring following early-life antibiotic exposure. We detail DNA extraction and 16S rRNA gene sequencing to longitudinally profile the composition of the fecal microbiota. Finally, we discuss how to address potential confounders such as the stochastic recolonization of the gut microbiota following antibiotic exposure. For complete details on the use and execution of this protocol, please refer to Lynn et al. (2021).
我们使用小鼠模型来呈现这个方案，以评估早期生活中抗生素暴露对哺乳动物寿命和肠道微生物组随时间的组成的影响。我们描述了早期生活中抗生素暴露后进行的纵向粪便采样和健康监测。我们详细介绍了 DNA 提取和 16S rRNA 基因测序，以纵向分析粪便微生物组的组成。最后，我们讨论了如何解决潜在的混杂因素，例如抗生素暴露后肠道微生物组的随机再定植。有关该方案使用和执行的完整详细信息，请参阅 Lynn 等人（2021 年）。
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