鼠粪便样本细菌微生物组分析的微生物 DNA 分离、16S rRNA 扩增子测序和生物信息学分析。
Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples.
机构信息
The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC 3010, Australia.
The Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC 3010, Australia.
出版信息
STAR Protoc. 2022 Oct 21;3(4):101772. doi: 10.1016/j.xpro.2022.101772. eCollection 2022 Dec 16.
Fecal samples are frequently used to characterize bacterial populations of the gastrointestinal tract. A protocol is provided to profile gut bacterial populations using rodent fecal samples. We describe the optimal procedures for collecting rodent fecal samples, isolating genomic DNA, 16S rRNA gene V4 region sequencing, and bioinformatic analyses. This protocol includes detailed instructions and example outputs to ensure accurate, reproducible results and data visualization. Comprehensive troubleshooting and limitation sections address technical and statistical issues that may arise when profiling microbiota. For complete details on the use and execution of this protocol, please refer to Gubert et al. (2022).
粪便样本常用于描述胃肠道的细菌种群。本方案提供了一种使用啮齿动物粪便样本分析肠道细菌种群的方法。我们描述了收集啮齿动物粪便样本、分离基因组 DNA、16S rRNA 基因 V4 区测序和生物信息学分析的最佳程序。本方案包括详细的说明和示例输出，以确保准确、可重复的结果和数据可视化。全面的故障排除和局限性部分解决了在分析微生物组时可能出现的技术和统计问题。有关使用和执行本方案的完整详细信息，请参考 Gubert 等人（2022 年）。
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