Riedel H H, Langenbucher H, Mettler L
J Reprod Med. 1986 Jul;31(7):605-8.
The mouse oocyte was used to determine whether T-mycoplasma or Escherichia coli affects the fertilization and embryonal development of oocytes. Ureaplasma urealyticum (T-mycoplasma) in the mouse oocyte system had little influence on the fertilization rates achieved in vitro; however, in all the experiments there was a marked reduction of embryonal development after in vitro fertilization (IVF) of spermatozoa infected with Ureaplasma. Even in oocytes incubated with Ureaplasma only after having reached the two-cell stage, there was reduced development into blastocysts. Changes in the Ureaplasma concentration and incubation time had no major influence on the development into blastocysts. During IVF of mouse oocytes with spermatozoa infected with E. coli concentrations of 10(3)/mL, there were no changes in the fertilization or culture rate as compared to the control group. However, after insemination with 10(6) bacteria/milliliter there was no change in the IVF rates in the mouse oocyte; however, the culture rates were considerably reduced (19.5% as compared to 64.4% in the control group). Whether there are additional changes in the pregnancy rate after the transfer of blastocysts obtained after insemination of oocytes with infected spermatozoa remains a matter for future investigation.
利用小鼠卵母细胞来确定T支原体或大肠杆菌是否会影响卵母细胞的受精和胚胎发育。在小鼠卵母细胞系统中，解脲脲原体（T支原体）对体外受精率影响不大；然而，在所有实验中，用感染了解脲脲原体的精子进行体外受精（IVF）后，胚胎发育显著减少。即使在卵母细胞达到二细胞期后才与解脲脲原体一起孵育，发育成囊胚的情况也会减少。解脲脲原体浓度和孵育时间的变化对发育成囊胚没有重大影响。在用浓度为10³/mL的感染大肠杆菌的精子对小鼠卵母细胞进行IVF期间，与对照组相比，受精率或培养率没有变化。然而，在用10⁶个细菌/毫升授精后，小鼠卵母细胞的IVF率没有变化；然而，培养率显著降低（对照组为64.4%，相比之下为19.5%）。用感染精子对卵母细胞授精后获得的囊胚进行移植后，妊娠率是否还有其他变化仍有待进一步研究。
