Huyser C, Fourie F L, Oosthuizen M, Neethling A
Department of Obstetrics and Gynaecology, University of Pretoria, Pretoria, RSA.
J In Vitro Fert Embryo Transf. 1991 Oct;8(5):260-4. doi: 10.1007/BF01139781.
Semen samples from 183 consecutive unselected men participating in an in vitro fertilization program were retrospectively studied to determine the bacterial and fungal contamination rate before and after antibiotic treatment. To ascertain the influence of semen preparation (wash and swim-up method) on the incidence of microorganisms, semen from 102 male patients was studied before and after swimup. Antimicrobial treatment by prescription of antibiotics decreased the incidence of pathogens by 16.3% (P less than 0.0001). Semen processing was more effective by ridding 57.4% of semen samples of microbial contaminants (P less than 0.0001). When infection of culture media was observed during routine microscopy, all infected oocytes were degenerated, without evidence of fertilization or pronuclei.
对参与体外受精项目的183名连续入选的未选择男性的精液样本进行回顾性研究，以确定抗生素治疗前后的细菌和真菌污染率。为确定精液处理（洗涤和上游法）对微生物发生率的影响，对102名男性患者的精液在进行上游法处理前后进行了研究。按处方使用抗生素进行抗菌治疗使病原体发生率降低了16.3%（P<0.0001）。精液处理在清除57.4%的精液样本中的微生物污染物方面更有效（P<0.0001）。在常规显微镜检查期间观察到培养基感染时，所有受感染的卵母细胞均发生退化，没有受精或原核形成的迹象。
