Young Neil D, Kinkar Liina, Stroehlein Andreas J, Korhonen Pasi K, Stothard J Russell, Rollinson David, Gasser Robin B
Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, Australia.
Department of Tropical Disease Biology, Liverpool School of Tropical Medicine, Liverpool, UK.
Curr Res Parasitol Vector Borne Dis. 2021 Feb 19;1:100017. doi: 10.1016/j.crpvbd.2021.100017. eCollection 2021.
Many freshwater snails of the genus act as intermediate hosts in the life-cycles of schistosomes in Africa and adjacent regions. Currently, 37 species of representing four groups are recognised. The mitochondrial cytochrome oxidase subunit 1 (1) gene has shown utility for identifying and differentiating species and groups, but taxonomic relationships based on genetic data are not entirely consistent with those inferred using morphological and biological features. To underpin future systematic studies of members of the genus, we characterised here the mitochondrial genome of (from a defined laboratory strain) using a combined second- and third-generation sequencing and informatics approach, enabling taxonomic comparisons with other planorbid snails for which mitochondrial (mt) genomes were available. Analyses showed consistency in gene order and length among mitochondrial genomes of representative planorbid snails, with the lowest and highest nucleotide diversities being in the cytochrome oxidase and nicotinamide dehydrogenase subunit genes, respectively. This first mt genome for a representative of the genus should provide a useful resource for future investigations of the systematics, population genetics, epidemiology and/or ecology of and related snails. The sequencing and informatic workflow employed here should find broad applicability to a range of other snail intermediate hosts of parasitic trematodes.
在非洲及邻近地区，许多[属名]淡水蜗牛在血吸虫的生命周期中充当中间宿主。目前，已识别出代表四个类群的37种[属名]蜗牛。线粒体细胞色素c氧化酶亚基1（COI）基因已被证明可用于识别和区分[属名]蜗牛的物种和类群，但基于遗传数据的分类关系与利用形态学和生物学特征推断的关系并不完全一致。为了支持该属成员未来的系统研究，我们在这里使用第二代和第三代测序及信息学相结合的方法对[属名]（来自一个明确的实验室菌株）的线粒体基因组进行了表征，以便与其他可获得线粒体（mt）基因组的扁卷螺进行分类比较。分析表明，代表性扁卷螺线粒体基因组之间的基因顺序和长度具有一致性，细胞色素c氧化酶基因和烟酰胺脱氢酶亚基基因的核苷酸多样性分别最低和最高。该属代表性物种的首个线粒体基因组应为未来对[属名]及相关蜗牛的系统学、群体遗传学、流行病学和/或生态学研究提供有用资源。这里采用的测序和信息学工作流程应广泛适用于一系列其他寄生吸虫的蜗牛中间宿主。
