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巴西用于检测马匹抗抗体的缓冲抗原和快速血清凝集试验(BA/Te)的评估

Evaluation of buffered antigen and rapid serum agglutination test (BA/Te) for the detection of anti- antibodies in horses in Brazil.

作者信息

Reck Carolina, Menin Álvaro, Batista Franciane, Meira Santos Patricia Oliveira, Miletti Luiz Claudio

机构信息

Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.

VERTÁ - Laboratory of Veterinary Diagnostic / Institute of Veterinary Research and Diagnostic, Av. Lions, 1286 - Bairro Nossa Sra. Aparecida, Curitibanos, SC 89520-000, Brazil.

出版信息

Curr Res Parasitol Vector Borne Dis. 2021 Apr 24;1:100024. doi: 10.1016/j.crpvbd.2021.100024. eCollection 2021.

DOI:10.1016/j.crpvbd.2021.100024
PMID:35284895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8906065/
Abstract

Surra is an infectious disease caused by , which affects a large number of domestic and wild animal species. Infection control is based on rapid diagnosis followed by treatment of sick animals. This study aimed to evaluate a buffered antigen and rapid serum agglutination test (BA/Te) for the detection of anti- antibodies in serum samples of horses. For this purpose, 445 serum samples from horses were evaluated and the results compared with the diagnosis by CATT/. Our data show a sensitivity of 92%, specificity of 91% and a degree of agreement kappa () of 0.82 (95% CI: 0.771-0.877,  < 0.01) between BA/Te and CATT/. Antigen specificity was also evaluated against reactive serum for other infectious agents circulating in equine herds. In conclusion, our findings show that BA/Te has the potential to be a practical and quick screening method for the detection of anti- antibodies in horses.

摘要

苏拉病是由……引起的一种传染病,影响大量家畜和野生动物物种。感染控制基于对患病动物的快速诊断并随后进行治疗。本研究旨在评估一种缓冲抗原和快速血清凝集试验(BA/Te)用于检测马血清样本中的抗……抗体。为此,对445份马血清样本进行了评估,并将结果与补体结合试验(CATT/……)诊断结果进行比较。我们的数据显示BA/Te与CATT/……之间的敏感性为92%,特异性为91%,kappa(κ)一致性程度为0.82(95%置信区间:0.771 - 0.877,P < 0.01)。还针对马群中循环的其他传染原的反应性血清评估了抗原特异性。总之,我们的研究结果表明BA/Te有潜力成为一种实用且快速的检测马抗……抗体的筛查方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60f2/8906065/f28cd5e9ad70/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60f2/8906065/f28cd5e9ad70/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60f2/8906065/f28cd5e9ad70/ga1.jpg

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Trypanosoma evansi and surra: a review and perspectives on transmission, epidemiology and control, impact, and zoonotic aspects.伊氏锥虫病和苏拉病:传播、流行病学和控制、影响以及人畜共患方面的综述和展望。
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Trypanosoma evansi and surra: a review and perspectives on origin, history, distribution, taxonomy, morphology, hosts, and pathogenic effects.
伊氏锥虫和苏拉病:起源、历史、分布、分类学、形态学、宿主和致病作用的综述及展望。
Biomed Res Int. 2013;2013:194176. doi: 10.1155/2013/194176. Epub 2013 Aug 19.
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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis.
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