巴西用于检测马匹抗抗体的缓冲抗原和快速血清凝集试验（BA/Te）的评估
Evaluation of buffered antigen and rapid serum agglutination test (BA/Te) for the detection of anti- antibodies in horses in Brazil.
作者信息
Reck Carolina, Menin Álvaro, Batista Franciane, Meira Santos Patricia Oliveira, Miletti Luiz Claudio
机构信息
Laboratório de Bioquímica de Hemoparasitas e Vetores, Departamento de Produção Animal e Alimentos, Centro de Ciências Agroveterinárias, Universidade do Estado de Santa Catarina, Av. Luiz de Camões, 2090 Lages, SC, Brazil.
VERTÁ - Laboratory of Veterinary Diagnostic / Institute of Veterinary Research and Diagnostic, Av. Lions, 1286 - Bairro Nossa Sra. Aparecida, Curitibanos, SC 89520-000, Brazil.
出版信息
Curr Res Parasitol Vector Borne Dis. 2021 Apr 24;1:100024. doi: 10.1016/j.crpvbd.2021.100024. eCollection 2021.
Surra is an infectious disease caused by , which affects a large number of domestic and wild animal species. Infection control is based on rapid diagnosis followed by treatment of sick animals. This study aimed to evaluate a buffered antigen and rapid serum agglutination test (BA/Te) for the detection of anti- antibodies in serum samples of horses. For this purpose, 445 serum samples from horses were evaluated and the results compared with the diagnosis by CATT/. Our data show a sensitivity of 92%, specificity of 91% and a degree of agreement kappa () of 0.82 (95% CI: 0.771-0.877, < 0.01) between BA/Te and CATT/. Antigen specificity was also evaluated against reactive serum for other infectious agents circulating in equine herds. In conclusion, our findings show that BA/Te has the potential to be a practical and quick screening method for the detection of anti- antibodies in horses.
苏拉病是由……引起的一种传染病，影响大量家畜和野生动物物种。感染控制基于对患病动物的快速诊断并随后进行治疗。本研究旨在评估一种缓冲抗原和快速血清凝集试验（BA/Te）用于检测马血清样本中的抗……抗体。为此，对445份马血清样本进行了评估，并将结果与补体结合试验（CATT/……）诊断结果进行比较。我们的数据显示BA/Te与CATT/……之间的敏感性为92%，特异性为91%，kappa（κ）一致性程度为0.82（95%置信区间：0.771 - 0.877，P < 0.01）。还针对马群中循环的其他传染原的反应性血清评估了抗原特异性。总之，我们的研究结果表明BA/Te有潜力成为一种实用且快速的检测马抗……抗体的筛查方法。
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