Rozenberg-Arska M, Porsius J C, Jaarsma E Y, Verhoef J
J Med Microbiol. 1986 Sep;22(2):143-9. doi: 10.1099/00222615-22-2-143.
The effect of human serum on Escherichia coli was studied with serum-sensitive and serum-resistant strains. The bactericidal effect of human serum on serum-sensitive strains of E. coli depended on the activation of the classical complement pathway. The role of activation of the alternative pathway was less important. After incubation in sub-bactericidal concentrations of serum these strains were also easily phagocytosed by polymorphonuclear leukocytes (PMNL). Strains of E. coli of certain O-types required not only an intact classical pathway but also the presence of specific antibodies for effective killing by serum and effective phagocytosis by PMNL, despite rapid activation of complement and rapid deposition of C3 on the bacterial surface in the absence of antibody. Capsulate strains O1K1 and O78K80 resisted the bactericidal effect of serum even in the presence of specific antibodies; phagocytosis by PMNL only occurred after opsonisation with specific antibodies.
利用血清敏感型和血清抗性型菌株研究了人血清对大肠杆菌的作用。人血清对血清敏感型大肠杆菌菌株的杀菌作用取决于经典补体途径的激活。替代途径激活的作用不太重要。在亚杀菌浓度的血清中孵育后，这些菌株也很容易被多形核白细胞（PMNL）吞噬。某些O型大肠杆菌菌株不仅需要完整的经典途径，还需要特定抗体的存在，才能通过血清有效杀伤并被PMNL有效吞噬，尽管在没有抗体的情况下补体能快速激活且C3能快速沉积在细菌表面。包膜菌株O1K1和O78K80即使在存在特异性抗体的情况下也能抵抗血清的杀菌作用；只有在用特异性抗体进行调理后，PMNL才能进行吞噬作用。
