Chin J K, Rong G H, Scharff J E, Sindelar W F
J Natl Cancer Inst. 1986 Sep;77(3):599-604. doi: 10.1093/jnci/77.3.599.
A murine monoclonal antibody, CHIP, has been prepared against a human pancreatic carcinoma cell line, SHAW. With the use of the avidin-biotin immunoperoxidase technique, the CHIP antibody detected an antigen found in 11 of 20 fixed tissue sections of tumors obtained from patients with pancreatic carcinoma. The antibody also detected the antigen in 25 of 26 colon carcinoma specimens, 4 of 6 gastric carcinoma specimens, and 1 of 2 esophageal adenocarcinoma specimens. The antigen was also found in normal proximal jejunum and colon and in small amounts in pancreatic islets and parathyroid. There was no reactivity with normal pancreatic ductal or acinar cells or with mesenchymal tissues.
已制备出一种针对人胰腺癌细胞系SHAW的鼠单克隆抗体CHIP。利用抗生物素蛋白-生物素免疫过氧化物酶技术，CHIP抗体在取自胰腺癌患者的20份肿瘤固定组织切片中的11份中检测到一种抗原。该抗体在26份结肠癌标本中的25份、6份胃癌标本中的4份以及2份食管腺癌标本中的1份中也检测到了该抗原。在正常近端空肠和结肠中也发现了这种抗原，在胰岛和甲状旁腺中含量较少。正常胰腺导管或腺泡细胞以及间充质组织无反应性。
