National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Central 3, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8563, Japan.
Anal Sci. 2022 Apr;38(4):657-664. doi: 10.1007/s44211-022-00080-z. Epub 2022 Feb 28.
Raman scattered light is generated by the interaction of irradiated light with molecular vibrations, and it provides molecular information. Although Raman shifts of peaks in the low-frequency region (< 200 cm) provide useful information related to molecular structures, there are no reliable materials available for calibrating Raman spectrometers in this region. In this study, we chose high-purity L-cystine (NMIJ CRM 6025-a) and used it to reliably evaluate Raman shifts of peaks with uncertainty by a combination of HeNe laser and Ne emission lines. To evaluate their uncertainty, we considered the uncertainty originating from wavenumbers of Ne emission lines and HeNe laser, evaluation of wavenumber for peak-tops and changes in temperature. The obtained Raman shifts of eight peaks from 9 to 160 cm and their uncertainty were 0.3 cm or 0.4 cm and these values were validated with the results using a 532 nm laser.
拉曼散射光由照射光与分子振动的相互作用产生，并提供分子信息。虽然低频区（<200 cm）的峰的拉曼位移提供了与分子结构相关的有用信息，但在该区域没有可靠的材料可用于校准拉曼光谱仪。在这项研究中，我们选择了高纯度 L-胱氨酸（NMIJ CRM 6025-a），并结合氦氖激光和氖发射线使用它来可靠地评估具有不确定度的峰的拉曼位移。为了评估它们的不确定性，我们考虑了源自氖发射线和氦氖激光的波数、峰顶点的波数评估以及温度变化的不确定性。从 9 到 160 cm 获得的 8 个峰的拉曼位移及其不确定度为 0.3 cm 或 0.4 cm，使用 532 nm 激光验证了这些值的结果。
