Department of Otolaryngology, Hualien Tzu Chi Hospital, Medical Foundation, Hualien, Taiwan.
Department of otolaryngology Head and Neck Surgery, School of Medicine, Chi University, Hualien, Taiwan.
Acta Otolaryngol. 2022 Feb;142(2):127-131. doi: 10.1080/00016489.2022.2038796. Epub 2022 Mar 15.
Cisplatin is a chemotherapy drug that can induce sensorineural hearing loss. At present, no otoprotective agent is approved for use.
This study investigated the optimal concentration of intratympanic N-acetylcysteine (NAC) to prevent cisplatin-induced ototoxicity in a guinea pig model.
Guinea pigs ( = 64) were treated with a single intratympanic injection containing different NAC concentrations or saline (control) 3 days prior to intraperitoneal injection with cisplatin. The threshold change in the auditory brainstem response was assessed.
Four weeks after intraperitoneal cisplatin injection, only the group that received 2% NAC exhibited significant otoprotection ( < .05) compared with the control. Otoprotection was observed at all the frequencies tested (1k, 2k, 4k, and 8k Hz). The 2% NAC group also exhibited significant otoprotection ( < .05) compared with the other NAC groups (at 1k, 2k, 4k, and 8k Hz). The 4% NAC group exhibited significantly reduced hearing capacity ( < .05) in the fourth week compared with controls.
Intratympanic NAC administration is an efficient and safe means of preventing cisplatin-induced ototoxicity. In our animal model, the optimal intratympanic NAC concentration was 2%; concentrations of 4% loss of otoprotection.
顺铂是一种化疗药物，可引起感音神经性听力损失。目前，尚无批准用于预防顺铂耳毒性的耳保护剂。
本研究旨在探讨鼓室内给予 N-乙酰半胱氨酸（NAC）的最佳浓度，以预防豚鼠模型中顺铂诱导的耳毒性。
豚鼠（ = 64）在腹腔注射顺铂前 3 天接受不同 NAC 浓度的鼓室内注射或生理盐水（对照）治疗。评估听觉脑干反应的阈值变化。
腹腔注射顺铂 4 周后，仅接受 2% NAC 治疗的组与对照组相比表现出显著的耳保护作用（ < .05）。在所有测试频率（1k、2k、4k 和 8k Hz）均观察到耳保护作用。与其他 NAC 组相比（在 1k、2k、4k 和 8k Hz），2% NAC 组也表现出显著的耳保护作用（ < .05）。与对照组相比，4% NAC 组在第 4 周时听力明显下降（ < .05）。
鼓室内给予 NAC 是预防顺铂诱导的耳毒性的有效且安全的方法。在我们的动物模型中，最佳的鼓室内 NAC 浓度为 2%；浓度为 4%则失去耳保护作用。
