Ikedo M, Yabuuchi E
Microbiol Immunol. 1986;30(5):413-23. doi: 10.1111/j.1348-0421.1986.tb02967.x.
The occurrence and viable counts of Legionella pneumophila in acid-treated water samples of 62 cooling towers on the main island of Japan were determined by inoculating them onto plates of Wadowsky-Yee-Okuda (WYO) agar medium. WYO plate cultures of 39 (63%) of the samples yielded L. pneumophila with viable counts ranging from 10 to 10(4) colony-forming units per 100 ml. Of the L. pneumophila isolates, 157 were serologically identified as serogroup 1, and the remaining 21 were agglutinated by serogroup 3 (2 strains) and serogroup 6 (19 strains) antisera. In each culture-positive water sample, the pH and the number of other bacteria were found not be statistically significantly correlated with the viable counts of L. pneumophila. However, a higher rate of recovery of L. pneumophila was obtained with the water samples with a smaller number of other bacteria. Practical use of commercially available antialgal or antimicrobial agents was found not to be significantly effective for controlling the occurrence and growth of L. pneumophila in cooling tower water.
通过将日本主岛上62座冷却塔的酸处理水样接种到Wadowsky - Yee - Okuda（WYO）琼脂培养基平板上，测定嗜肺军团菌的发生率和活菌数。39个（63%）样本的WYO平板培养物培养出嗜肺军团菌，活菌数为每100毫升10至10⁴个菌落形成单位。在嗜肺军团菌分离株中，157株经血清学鉴定为血清1型，其余21株被血清3型（2株）和血清6型（19株）抗血清凝集。在每个培养阳性的水样中，发现pH值和其他细菌数量与嗜肺军团菌的活菌数无统计学显著相关性。然而，其他细菌数量较少的水样中嗜肺军团菌的回收率较高。发现市售的抗藻剂或抗菌剂在控制冷却塔水中嗜肺军团菌的发生和生长方面效果不显著。
