Zhdan-Pushkina S M, Verbitskaia N B, Kondrat'eva L D
Mikrobiologiia. 1986 May-Jun;55(3):357-61.
The resting cells of different E. coli cells remained viable after their heating at 48 degrees C for 30 min. The activity of their succinate dehydrogenase (SDH) (EC 1.3.99.1) was not more than 50% of the control one. When the cells were inoculated after a heat stress into a peptone medium, they started to grow at a high rate. However, their maximal specific growth rate mu and the overall biomass yield were less than in the control. The SDH activity of the cells reached the original level by the end of the logarithmic growth phase. This did not happen when the cells were incubated in 0.14 M NaCl for a time necessary for the culture to reach the end of the logarithmic growth phase. The SDH activity (in absolute values) of cell-free extracts was not greater than 35% of the cell SDH activity. The SDH activity of the cell-free extracts did not change after their heating at 48 degrees C. The SDH activity of E. coli cells is recommended to be used as a parameter indicative of their stress state.
不同大肠杆菌细胞的静止细胞在48℃加热30分钟后仍保持活力。其琥珀酸脱氢酶（SDH）（EC 1.3.99.1）的活性不超过对照的50%。当热应激后的细胞接种到蛋白胨培养基中时，它们开始快速生长。然而，它们的最大比生长速率μ和总生物量产量低于对照。细胞的SDH活性在对数生长期结束时达到原始水平。当细胞在0.14 M NaCl中孵育至培养物达到对数生长期结束所需的时间时，情况并非如此。无细胞提取物的SDH活性（绝对值）不大于细胞SDH活性的35%。无细胞提取物在48℃加热后其SDH活性没有变化。建议将大肠杆菌细胞的SDH活性用作指示其应激状态的参数。
