Elisashvili V I, Soshnikova M V
Mikrobiologiia. 1984 May-Jun;53(3):483-8.
The heating of Pseudomonas geniculata 338 at an elevated temperature causes a heat stress in the culture. The extent of the stress depends on the temperature and duration of heating. The incubation of the bacterium at 40 and 45 degrees C did not inhibit its growth after 30 min of heating, and no essential quantities of intracellular compounds absorbing at 260 nm were lost (E260 increased by 12-19%). When the bacterium was heated at 50 degrees C for the same period of time, a three-hour lag-phase appeared during the subsequent cultivation of the bacterium whereas. E260 rose by a factor of 1.7. The resistance of the bacterium to heating depended on the physiological state of the culture: cells at the logarithmic growth phase were most susceptible to heating while the bacterium became more resistant to heating in the course of aging. The addition of NaCl at a concentration of 1.5% or of 10(-3)-10(-4) M EDTA to the reparation medium makes it possible to estimate the population of bacterial cells in the state of stress.
对膝状假单胞菌338进行高温加热会在培养物中引发热应激。应激程度取决于加热温度和持续时间。该细菌在40℃和45℃下培养30分钟后加热并未抑制其生长，且在260nm处吸收的细胞内化合物也未损失大量（E260增加了12 - 19%）。当细菌在50℃下加热相同时间时，在随后的细菌培养过程中出现了三小时的滞后期，而E260升高了1.7倍。细菌对加热的抗性取决于培养物的生理状态：对数生长期的细胞对加热最敏感，而随着老化过程细菌对加热的抗性增强。向修复培养基中添加浓度为1.5%的NaCl或10⁻³ - 10⁻⁴M的EDTA，能够估计处于应激状态的细菌细胞数量。
