Department of Histology and Embryology, Institute of Stem Cell and Tissue Engineering, School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, China.
Centre for Reproductive Medicine, Affiliated Maternity and Child Health Hospital of Anhui Medical University, Anhui Province Maternity and Child Health Hospital, Hefei, Anhui, China.
Reprod Toxicol. 2022 Apr;109:53-60. doi: 10.1016/j.reprotox.2022.03.001. Epub 2022 Mar 12.
Menstrual blood mesenchymal stem cell (MBMSC) is a potential cell source for effective therapy for intrauterine adhesion (IUA). Collagen scaffold (CS) loaded with mesenchymal stem cells promotes endometrial regeneration in IUA model animals. However, role of combination of MBMSCs and CS in IUA therapy remains elusive. In particular, transplantation of MBMSCs over a long period of time requires more in-depth research. Here in this study, transplantation of human MBMSCs loaded on CS was applied for therapy for a long term rat IUA model. A rat IUA model characterized by lower number of endometrial glands and increased fibrosis was established. At 90 days after transplantation of the human MBMSC-loaded CS, expression of HuNu, a human protein, was identified in the uteri of the transplanted IUA model rats. The transplantation increased the number of endometrial glands and decreased the fibrotic areas significantly. Moreover, transplantation of the human MBMSC-loaded CS decreased the Collagen I and increased the CK 18 significantly. Immunoblotting assay results further proved the downregulation of Collagen I and the upregulation of CK 18. Together, endometrium regeneration promoted by human MBMSC-loaded CS was demonstrated in a long term rat model of IUA, shedding a new light on the role of human MBMSCs in the therapy for IUA.
经血间充质干细胞（MBMSC）是一种有潜力的细胞来源，可有效治疗宫腔粘连（IUA）。负载间充质干细胞的胶原支架（CS）促进 IUA 模型动物的子宫内膜再生。然而，MBMSCs 与 CS 的联合在 IUA 治疗中的作用仍不清楚。特别是，MBMSCs 的长期移植需要更深入的研究。在本研究中，将负载人 MBMSCs 的 CS 移植用于长期大鼠 IUA 模型的治疗。建立了一种以子宫内膜腺体数量减少和纤维化增加为特征的大鼠 IUA 模型。在移植负载人 MBMSC 的 CS 后 90 天，在移植的 IUA 模型大鼠的子宫中鉴定出人蛋白 HuNu 的表达。移植增加了子宫内膜腺体的数量，并显著减少了纤维化区域。此外，负载人 MBMSC 的 CS 移植显著降低了 Collagen I 并增加了 CK 18。免疫印迹分析结果进一步证明了 Collagen I 的下调和 CK 18 的上调。总之，在长期大鼠 IUA 模型中证明了负载人 MBMSC 的 CS 促进子宫内膜再生，为 MBMSCs 在 IUA 治疗中的作用提供了新的认识。
