Chen Fang, Gong Yingxin, Jiang Ninghong, Xiao Jingjing, Wang Yaping, Chen Limei, Sui Long
Department of Gynecology and Obstetrics, Obstetrics and Gynecology Hospital of Fudan University Shanghai, China.
Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai, China.
Am J Transl Res. 2022 Sep 15;14(9):6712-6725. eCollection 2022.
This study aimed to verify the role of basic fibroblast growth factor (bFGF)-bone mesenchymal stem cells (BMSCs) loaded on collagen scaffolds for the repair of injured endometrium.
We established an intrauterine adhesion (IUA) model in rats by endometrial resection and implanted BMSCs and bFGF-BMSCs loaded on collagen scaffolds into uteri. A total of 100 IUA model rats were divided into five groups: the control group, scaffold group, BMSC+scaffold group, vector-BMSC group, and bFGF-BMSC+scaffold group. The rats were sacrificed on the 3, 7, 15, and 45 days. The endometrium thickness, number of glands, and microvascular density were measured by hematoxylin and eosin staining, Masson staining, and immunohistochemistry staining of CD31. The expression of bFGF, vascular endothelial growth factor (VEGF), vimentin, and Ki67 was assayed by immunohistochemistry staining.
The bFGF-BMSCs loaded on the collagen scaffold significantly increased the endometrial thickness, gland number, and microvascular density, which greatly promoted the regeneration of the injured endometrium (P<0.0001). In addition, the expression levels of bFGF, VEGF, vimentin, and Ki67 were significantly higher in the bFGF-BMSC+scaffold group than in the BMSC+scaffold group (P<0.05).
Our findings indicated that bFGF-BMSCs loaded on collagen scaffolds have the ability to prompt the regeneration of the endometrium after injury, contributing to a better understanding of stem cell treatment for intrauterine adhesion.
本研究旨在验证负载于胶原支架上的碱性成纤维细胞生长因子（bFGF）-骨髓间充质干细胞（BMSCs）在损伤子宫内膜修复中的作用。
我们通过子宫内膜切除术建立大鼠宫腔粘连（IUA）模型，并将负载于胶原支架上的BMSCs和bFGF-BMSCs植入子宫。总共100只IUA模型大鼠被分为五组：对照组、支架组、BMSC+支架组、载体-BMSC组和bFGF-BMSC+支架组。在第3、7、15和45天处死大鼠。通过苏木精-伊红染色、Masson染色和CD31免疫组织化学染色测量子宫内膜厚度、腺体数量和微血管密度。通过免疫组织化学染色检测bFGF、血管内皮生长因子（VEGF）、波形蛋白和Ki67的表达。
负载于胶原支架上的bFGF-BMSCs显著增加了子宫内膜厚度、腺体数量和微血管密度，极大地促进了损伤子宫内膜的再生（P<0.0001）。此外，bFGF-BMSC+支架组中bFGF、VEGF、波形蛋白和Ki67的表达水平显著高于BMSC+支架组（P<0.05）。
我们的研究结果表明，负载于胶原支架上的bFGF-BMSCs具有促进损伤后子宫内膜再生的能力，有助于更好地理解宫腔粘连的干细胞治疗。
