Ishikawa K, Katakami H, Jansson J O, Frohman L A
Neuroendocrinology. 1986;43(5):537-42. doi: 10.1159/000124579.
The ontogenesis of growth hormone releasing hormone (GH-RH) containing neurons in the rat hypothalamus has been studied by immunohistochemistry, using a specific anti-rat GH-RH serum. Immunoreactive fibers were first detected in the prospective median eminence on day 18 of gestation. During the subsequent 3 days, they rapidly increased in distribution and intensity of staining within this structure. On day 21, positive fibers were also visible in a plexus within the arcuate nucleus. In 1-day-old rats treated with colchicine, positive perikarya were distributed in several hypothalamic nuclei, including the arcuate nucleus, dorsomedial nucleus, basal lateral hypothalamus, and perifornical region. The distribution was similar to that previously described in adult rats. The intensity of staining in the various hypothalamic regions increased during early postnatal life to levels nearly comparable to those in adult rats by 30 days. These findings showing the early appearance of GH-RH-positive terminals in the median eminence and the wide distribution of the perikarya at an early stage of postnatal life support the view that hypothalamic GH-RH serves an important role in the regulation of growth hormone secretion during late prenatal and early neonatal periods.
利用一种特异性抗大鼠生长激素释放激素（GH-RH）血清，通过免疫组织化学方法研究了大鼠下丘脑内含有GH-RH的神经元的个体发生。在妊娠第18天，在前庭正中隆起首次检测到免疫反应性纤维。在随后的3天里，它们在该结构内的分布和染色强度迅速增加。在第21天，在弓状核内的一个神经丛中也可见到阳性纤维。在用秋水仙碱处理的1日龄大鼠中，阳性核周体分布在几个下丘脑核内，包括弓状核、背内侧核、下丘脑基底外侧和穹窿周区。其分布与先前在成年大鼠中描述的相似。在出生后早期，各个下丘脑区域的染色强度增加，到30天时几乎达到成年大鼠的水平。这些结果表明，正中隆起中GH-RH阳性终末的早期出现以及出生后早期核周体的广泛分布支持了这样一种观点，即下丘脑GH-RH在产前后期和新生儿早期生长激素分泌的调节中起重要作用。
