VandePol C J, Leidy J W, Finger T E, Robbins R J
Neuroendocrinology. 1986;42(2):143-7. doi: 10.1159/000124265.
Brains from 4 normal and 4 colchicine-treated rats were studied for the presence of growth hormone-releasing factor (GRF) using an antibody directed against rat hypothalamic GRF (rGRF). Noncolchicine-treated animals showed intense staining in the external layer of the median eminence. Rats pretreated with intraventricular colchicine for 48 h showed localization of GRF-containing cell bodies in the arcuate nucleus, the perifornical area, the lateral basal hypothalamic region and lateral to the ventromedial nucleus and the premammillary nucleus. This pattern of distribution is consistent with the postulated role of GRF in hypothalamic regulation of growth hormone secretion. Our results confirm the predominant localization of GRF perikarya in the arcuate nucleus of the rat which has been noted in studies using antibodies directed against both human pancreas GRF (hpGRF) and rGRF, and demonstrate a unique pattern of rGRF-positive cell bodies elsewhere in the hypothalamus. No rGRF perikarya or processes were seen outside the hypothalamus.
使用针对大鼠下丘脑生长激素释放因子（rGRF）的抗体，对4只正常大鼠和4只秋水仙碱处理大鼠的大脑进行研究，以检测生长激素释放因子（GRF）的存在情况。未用秋水仙碱处理的动物在正中隆起外层显示出强烈染色。经脑室内注射秋水仙碱预处理48小时的大鼠，其含GRF的细胞体定位于弓状核、穹窿周区、下丘脑外侧基底部区域以及腹内侧核和乳头前核外侧。这种分布模式与GRF在下丘脑调节生长激素分泌中的假定作用一致。我们的结果证实了在使用针对人胰腺GRF（hpGRF）和rGRF的抗体进行的研究中所观察到的，GRF核周体在大鼠弓状核中的主要定位，并证明了下丘脑其他部位rGRF阳性细胞体的独特模式。在下丘脑外未观察到rGRF核周体或突起。
