基于亮 RNA 适体靶标触发转录的 miRNA 检测新方法。
A novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer.
机构信息
Department of Chemical and Biomolecular Engineering (BK21 four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
出版信息
Chem Commun (Camb). 2022 Mar 29;58(26):4243-4246. doi: 10.1039/d1cc07095a.
We herein describe a novel method for miRNA detection based on target-triggered transcription of a light-up RNA aptamer (TTRApt), consequently producing a highly enhanced fluorescence signal through specific binding of a light-up RNA aptamer to the corresponding dye. Using this strategy, we successfully identified target miRNA down to 59.4 aM with excellent specificity.
我们在此描述了一种基于亮报告基因 RNA 适体（TTRApt）靶触发转录的新型 miRNA 检测方法，通过亮报告基因 RNA 适体与相应染料的特异性结合，从而产生高度增强的荧光信号。利用该策略，我们成功地以 59.4 aM 的低检测限且具有优异特异性的方式识别了靶 miRNA。
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