Ehrlich K C, Stewart E, Klein E
In Vitro. 1978 May;14(5):443-50. doi: 10.1007/BF02616106.
Glucose, lactic-acid, and oxygen metabolism of BHK and L929 cells on artificial capillary perfusion units have been studied using several different modes of perfusion. After 7 to 10 days, cells planted in the extracapillary compartment of culture units containing 80 to 150 fibers reached populations that used 0.073 +/- 0.025 mumol per min glucose and 0.76 +/- 0.26 microliter per min oxygen and excreted 0.078 +/- 0.038 mumol per min lactic acid. From these data it is estimated that these units contain approximately 2 x 10(7) cells. The metabolic rate of cultures perfused through the capillaries or through the extracapillary compartment was not affected significantly by change in flow rate except at perfusion flow rates less than or equal to 0.05 ml per min. The cell population, as measured by metabolic activity, did not increase significantly when the serum content of the medium was less than or equal to 1%. No major differences were found in glucose utilization rates of equal numbers of cells on artificial capillaries, on short-term suspension culture, or as monolayers in plastic flasks. Artificial capillary perfusion may provide a simple system for studying metabolism of mammalian cells in culture.
利用几种不同的灌注模式，对人工毛细血管灌注装置上的BHK细胞和L929细胞的葡萄糖、乳酸和氧代谢进行了研究。7至10天后，种植在含有80至150根纤维的培养装置毛细血管外隔室中的细胞群体，其葡萄糖利用速率为每分钟0.073±0.025微摩尔，氧消耗速率为每分钟0.76±0.26微升，乳酸分泌速率为每分钟0.078±0.038微摩尔。根据这些数据估计，这些装置中大约含有2×10⁷个细胞。通过毛细血管或毛细血管外隔室进行灌注的培养物的代谢速率，除了在灌注流速小于或等于每分钟0.05毫升时，不受流速变化的显著影响。当培养基中的血清含量小于或等于1%时，通过代谢活性测量的细胞群体没有显著增加。在人工毛细血管上、短期悬浮培养中或塑料培养瓶中的单层培养中，等量细胞的葡萄糖利用率没有发现重大差异。人工毛细血管灌注可为研究培养中的哺乳动物细胞代谢提供一个简单的系统。
