Singapore Institute of Manufacturing Technology, Singapore, Singapore.
Materials Science and Engineering School, Nanyang Technological University, Singapore, Singapore.
Crit Rev Biotechnol. 2023 May;43(3):433-464. doi: 10.1080/07388551.2022.2037503. Epub 2022 Mar 15.
Digital polymerase chain reaction (dPCR) technology has provided a new technique for molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and specificity over quantitative real-time PCRs (qPCR). Eight companies have offered commercial dPCR instruments: Fluidigm Corporation, Bio-Rad, RainDance Technologies, Life Technologies, Qiagen, JN MedSys Clarity, Optolane, and Stilla Technologies Naica. This paper discusses the working principle of each offered dPCR device and compares the associated: technical aspects, usability, costs, and current applications of each dPCR device. Lastly, up-and-coming dPCR technologies are also presented, as anticipation of how the dPCR device landscape may likely morph in the next few years.
数字聚合酶链反应（dPCR）技术为分子诊断提供了一种新的技术，具有更高的灵敏度、精度和特异性等优势，优于定量实时聚合酶链反应（qPCR）。目前有 8 家公司提供商业 dPCR 仪器：Fluidigm 公司、Bio-Rad、RainDance 技术公司、Life 技术公司、Qiagen、JN MedSys Clarity、Optolane 和 Stilla 技术公司 Naica。本文讨论了每种提供的 dPCR 仪器的工作原理，并比较了相关的：技术方面、可用性、成本和每个 dPCR 仪器的当前应用。最后，还介绍了即将出现的 dPCR 技术，以及对未来几年 dPCR 设备格局可能发生的变化的预期。
