Reproductive Medical Center, Peking University People's Hospital, Beijing, China.
Reproductive Medical Center, Peking University International Hospital, Beijing, China.
Zygote. 2022 Aug;30(4):487-494. doi: 10.1017/S0967199421000812. Epub 2022 Mar 16.
For individual cultures, findings on regulating embryo density by changing the microdrop volume are contradictory. The aim of this study was to investigate the relationship between embryo density and the developmental outcome of day 3 embryos after adjusting covariates. In total, 1196 embryos from 206 couples who had undergone fertilization treatment were analyzed retrospectively. Three embryo densities were used routinely, i.e. one embryo in a drop (30 μl/embryo), two embryos in a drop (15 μl/embryo) and three embryos in a drop (10 μl/embryo). Embryo quality on day 3 was evaluated, both the cell number of day 3 embryos and the proportion of successful implantations served as endpoints. Maternal age, paternal age, antral follicles and level of anti-Müllerian hormone, type of infertility, controlled ovarian stimulation protocol, length of stimulation, number of retrieved oocytes, number of zygotes (two pronuclei) and insemination type were covariates and adjusted. After adjusting fully for all covariates, the cell number of day 3 embryos was significantly increased by 0.40 (95% CI 0.00, 0.79; = 0.048) and 0.78 (95% CI 0.02, 1.54; = 0.044) in the 15 μl/embryo and 10 μl/embryo group separately, compared with the 30 μl/embryo group. The proportions of implanted embryos were 42.1%, 48.7% and 0.0% in the 30 μl/embryo, 15 μl/embryo and 10 μl/embryo groups respectively. There was no statistical significance ( = 0.22) between the 30 μl/embryo group and the 15 μl/embryo group. After adjusting for confounders that were significant in univariate analysis, embryo density was still not associated with day 3 embryo implantation potential ( > 0.05). In a 30-μl microdrop, culturing embryos with an embryo density of both 15 and 10 μl/embryo increased the cell number of day 3 embryos, which did not benefit embryo implanting potential, compared with individual culture of 30 μl/embryo.
对于个别文化而言，通过改变微滴体积来调节胚胎密度的研究结果存在矛盾。本研究旨在探讨调整协变量后第三天胚胎密度与胚胎发育结局的关系。本研究回顾性分析了 206 对接受体外受精治疗的夫妇的 1196 个胚胎。常规使用三种胚胎密度，即一滴一个胚胎（30 μl/胚胎）、一滴两个胚胎（15 μl/胚胎）和一滴三个胚胎（10 μl/胚胎）。第 3 天评估胚胎质量，以第 3 天胚胎的细胞数和成功着床率作为终点。调整协变量包括母亲年龄、父亲年龄、窦卵泡数和抗苗勒管激素水平、不孕类型、控制性卵巢刺激方案、刺激时间、取卵数、受精卵数（双原核）和授精类型。在完全调整所有协变量后，与 30 μl/胚胎组相比，15 μl/胚胎组和 10 μl/胚胎组第 3 天胚胎的细胞数分别显著增加 0.40（95%CI 0.00，0.79；=0.048）和 0.78（95%CI 0.02，1.54；=0.044）。30 μl/胚胎、15 μl/胚胎和 10 μl/胚胎组的着床胚胎比例分别为 42.1%、48.7%和 0.0%。30 μl/胚胎组与 15 μl/胚胎组之间无统计学差异（=0.22）。在单变量分析中，调整有统计学意义的混杂因素后，胚胎密度仍与第 3 天胚胎着床潜能无关（>0.05）。在 30 μl 的微滴中，与单独培养 30 μl/胚胎相比，培养 15 μl/胚胎和 10 μl/胚胎的胚胎密度均增加了第 3 天胚胎的细胞数，但并未提高胚胎着床潜能。
