Shi Cheng, Sun Tie Cheng, Meng Shi Hui, Wang Ping, Liang Rong, Duan Sheng Nan, Han Hong Jing, Liu Ya Nan, Shen Huan, Chen Xi
Reproductive Medical Center, Peking University People's Hospital, Peking University, Beijing 100044, China.
Department of Obstetrics and Gynecology, Peking University International Hospital, Beijing 102206, China.
Int J Endocrinol. 2022 Mar 12;2022:6368678. doi: 10.1155/2022/6368678. eCollection 2022.
Many studies have shown that embryo density has an impact on day-3 embryo-developmental outcomes; however, embryo density remains controversial in clinical practice. We aimed to evaluate the association between embryo density and day-3 embryo-developmental outcomes in real world with the largest sample size.
In 2018, we identified 10941 day-3 embryos from all female patients ( = 1568) in the study. The embryos were allocated to three embryonic densities: 30 l/embryo (individual culture), 15 l/embryo, and 10 l/embryo (group culture). The primary outcomes were cleaving speed, quality, and proportion of successful implantations. The generalized estimate equation (GEE) model was used both in the univariate analysis and multivariable logistic regression analyses to investigate the relationship between embryo density and embryo-developmental outcomes.
There were 3064, 5695, and 2182 embryos in the 30 l/embryo group, 15 l/embryo group, and 10 l/embryo group, respectively. The proportions of 7-10 cell embryos were 57.2%, 56.1%, and 58.3% in three densities with no statistical significance (=0.37), respectively. The proportions of morphologically good embryos were 20%, 20.3%, and 20% in three densities with no statistical significance (=0.85), respectively. Proportions of implanted embryos were 37.7%, 37.1%, and 27.8% with no statistical significance (=0.36), respectively. After adjustment for confounders, which were significant in the univariate analysis, the embryo density was still not associated with day-3 embryo-cleaving speed, day-3 embryo quality, and day-3 embryo-implanting potential (all > 0.05).
In a 30 l microdrop, the culturing embryos with embryo densities of 15, 10, and 30 l/embryo (from zygotes to day 3) had similar developmental outcomes. The embryo density had no impact on day-3 embryo development.
许多研究表明胚胎密度会影响第三天胚胎的发育结果；然而，胚胎密度在临床实践中仍存在争议。我们旨在以最大样本量评估现实世界中胚胎密度与第三天胚胎发育结果之间的关联。
2018年，我们从研究中的所有女性患者（n = 1568）中识别出10941个第三天胚胎。将这些胚胎分为三种胚胎密度：30 μl/胚胎（单独培养）、15 μl/胚胎和10 μl/胚胎（组培）。主要结局指标为分裂速度、质量和成功着床比例。在单因素分析和多变量逻辑回归分析中均使用广义估计方程（GEE）模型来研究胚胎密度与胚胎发育结果之间的关系。
30 μl/胚胎组、15 μl/胚胎组和10 μl/胚胎组分别有3064、5695和2182个胚胎。三种密度下7 - 10细胞胚胎的比例分别为57.2%、56.1%和58.3%，无统计学意义（P = 0.37）。三种密度下形态良好胚胎的比例分别为20%、20.3%和20%，无统计学意义（P = 0.85）。着床胚胎的比例分别为37.7%、37.1%和27.8%，无统计学意义（P = 0.36）。在对单因素分析中有显著意义的混杂因素进行调整后，胚胎密度仍与第三天胚胎分裂速度、第三天胚胎质量和第三天胚胎着床潜能无关（均P > 0.05）。
在30 μl微滴中，以15、10和30 μl/胚胎（从受精卵到第三天）的胚胎密度培养胚胎，其发育结果相似。胚胎密度对第三天胚胎发育无影响。
