Key Laboratory of Organ Regeneration and Transplantation of the Ministry of Education, Genetic Diagnosis Centre, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Oncol Rep. 2022 May;47(5). doi: 10.3892/or.2022.8300. Epub 2022 Mar 16.
The role of mast cells in colorectal cancer (CRC) has been an area of intense interest. Mast cell density is closely related to CRC development and prognosis. The identification of mast cell progenitors (MCps) in peripheral blood provides an opportunity to explore the frequency and distribution of mast cells in the circulation and tumour microenvironment of patients with CRC at different disease stages. The aim of the presents study was to investigate the changes of MCps and mast cells in CRC. Flow cytometry was used to measure the circulating frequency of MCps in 37 patients with CRC and 12 healthy control (HC) patients, and the frequency of mast cells in tissue from 15 patients with CRC and 7 patients with haemorrhoids. In the present study, lower levels of circulating MCps in patients with CRC were found, which was significantly related to CRC development. After surgery, the frequency of circulating MCps was significantly increased. However, the frequency of mast cells in tumour tissues was lower than that in adjacent normal tissues and compared with HC tissues and was not associated with CRC progression.
肥大细胞在结直肠癌（CRC）中的作用一直是研究的热点。肥大细胞密度与 CRC 的发生和预后密切相关。外周血中肥大细胞前体（MCp）的鉴定为探索不同疾病阶段 CRC 患者循环和肿瘤微环境中肥大细胞的频率和分布提供了机会。本研究旨在探讨 MCp 和肥大细胞在 CRC 中的变化。采用流式细胞术检测 37 例 CRC 患者和 12 例健康对照（HC）患者的循环 MCp 频率，以及 15 例 CRC 患者和 7 例痔疮患者组织中肥大细胞的频率。本研究发现，CRC 患者循环 MCp 水平较低，与 CRC 的发生明显相关。手术后，循环 MCp 的频率显著增加。然而，肿瘤组织中肥大细胞的频率低于相邻正常组织，与 HC 组织相比也较低，且与 CRC 的进展无关。
