Poulter L W, Campbell D A, Munro C, Janossy G
Scand J Immunol. 1986 Sep;24(3):351-7. doi: 10.1111/j.1365-3083.1986.tb02104.x.
Two monoclonal antibodies (MoAb), RFD1 and RFD7, have been used to investigate whether human macrophages and dendritic cells represent phenotypically distinct cell types. RFD7 recognizes a 77 kd antigen, and is specific for acid phosphatase positive tissue macrophages, while RFD1 recognizes a unique Class II antigen, which is associated with dendritic cells. With immunohistological/cytological methods it was found that neither of these reagents reacted with granulocytes, monocytes, or lymphocytes, with the exception that a small proportion (less than 20%) of B cells were stained with RFD1. In tissues, RFD7 reacted with mature macrophages only and did not stain Langerhans cells in the skin or the interdigitating (dendritic) cells of the T-cell zones of lymphoid tissue and the thymic medulla. Conversely, RFD1 appeared specific for the interdigitating (dendritic) cells and did not react with macrophage populations identified morphologically, geographically, and histochemically in any tissue studied. When peripheral blood monocytes (RFD1-, RFD7-) were matured in vitro, two distinct populations of RFD1+ RFD7- and RFD7+ RFD1- cells emerged. It is concluded that in normal tissues these two reagents identify phenotypic differences between macrophages and dendritic cells that may have functional significance.
两种单克隆抗体（MoAb），即RFD1和RFD7，已被用于研究人类巨噬细胞和树突状细胞在表型上是否代表不同的细胞类型。RFD7识别一种77kd的抗原，对酸性磷酸酶阳性的组织巨噬细胞具有特异性，而RFD1识别一种独特的II类抗原，该抗原与树突状细胞相关。通过免疫组织学/细胞学方法发现，这些试剂均不与粒细胞、单核细胞或淋巴细胞反应，但有一小部分（少于20%）的B细胞可被RFD1染色。在组织中，RFD7仅与成熟巨噬细胞反应，不染色皮肤中的朗格汉斯细胞或淋巴组织T细胞区和胸腺髓质的指状（树突状）细胞。相反，RFD1似乎对指状（树突状）细胞具有特异性，并且不与在所研究的任何组织中通过形态学、位置和组织化学鉴定的巨噬细胞群体反应。当外周血单核细胞（RFD1-，RFD7-）在体外成熟时，出现了两个不同的群体，即RFD1+RFD7-和RFD7+RFD1-细胞。结论是，在正常组织中，这两种试剂可识别巨噬细胞和树突状细胞之间可能具有功能意义的表型差异。
