Institute of Neuroscience, Basic Medical College, Kunming Medical University, Kunming 650500, China.
Department of Laboratory Animal Science, Kunming Medical University, Kunming 650500, China.
J Tradit Chin Med. 2022 Feb;42(1):96-101. doi: 10.19852/j.cnki.jtcm.20211012.001.
Yang-deficiency constitution (YADC) is a common unbalanced constitution that predisposes individuals to certain diseases. However, not all people with YADC manifest develop diseases. This calls for delineation of the underlying molecular mechanisms. Previous studies suggested that the gut microbiota and gene differential expression should be considered.
In the present study, we compared profiles of gut microbiota between four healthy YADC individuals and those of five healthy balanced constitution (BC) counterparts, based on 16S rRNA gene sequence analysis. Furthermore, YADC relevant genes identified by comparing 62 healthy YADC and 58 healthy BC individuals in total to perform intersection analysis, functional clustering and pathway enrichment analyses.
The levels of harmful gut microbiota (Prevotellaceae, LDA score > 4.0, P = 0.0141) and beneficial gut microbiota (Ruminococcaceae, LDA score > 4.0, P = 0.0025, Faecalibacterium, LDA score > 4.0, P = 0.0484) were both elevated in healthy YADC individuals. Also, we found that the specific metabolic pathway with 2, 6-Dichloro-p-hydroquinone 1, 2-Dioxygenase (PcpA) as the core in gut microbiota and the glutathione transferase activity has been enriched by YADC relevant genes in healthy YADC individuals were both responsible for the detoxification of halogenated aromatic hydrocarbon substances.
Both beneficial and harmful factors had been detected in healthy YADC individuals, functionally, they may have triggered homeostasis to maintain the health of individuals with YADC. The homeostasis may be maintained by beneficial and harmful factors from gut flora and genes. Future studies are expected to focus on halogenated aromatic hydrocarbons and their detoxification processes.
Yang-deficiency constitution（YADC）是一种常见的不平衡体质，易患某些疾病。然而，并非所有 YADC 患者都会发病。这就需要阐明潜在的分子机制。先前的研究表明，应考虑肠道微生物群和基因差异表达。
在本研究中，我们通过 16S rRNA 基因序列分析，比较了 4 名健康 YADC 个体和 5 名健康平衡体质（BC）个体的肠道微生物群谱。此外，我们还比较了 62 名健康 YADC 个体和 58 名健康 BC 个体，鉴定了 YADC 相关基因，并进行了交集分析、功能聚类和通路富集分析。
健康 YADC 个体的有害肠道微生物群（Prevotellaceae，LDA 评分>4.0，P=0.0141）和有益肠道微生物群（Ruminococcaceae，LDA 评分>4.0，P=0.0025，Faecalibacterium，LDA 评分>4.0，P=0.0484）水平均升高。此外，我们发现肠道微生物群中特定的代谢途径（以 2,6-二氯对苯二酚 1,2-双加氧酶（PcpA）为核心）和谷胱甘肽转移酶活性已被 YADC 相关基因富集，这些基因在健康 YADC 个体中负责卤代芳烃物质的解毒。
健康 YADC 个体中既存在有益因素，也存在有害因素，从功能上看，它们可能触发了内稳机制，维持了 YADC 个体的健康。这种内稳可能是由肠道菌群和基因中的有益和有害因素共同维持的。未来的研究有望集中在卤代芳烃及其解毒过程上。
