Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Department of Biotechnology, Periyar University, Salem, Tamil Nadu, India.
Environ Sci Pollut Res Int. 2023 Mar;30(15):42587-42595. doi: 10.1007/s11356-022-19661-6. Epub 2022 Mar 16.
The fall armyworm, Spodoptera frugiperda, has become a worldwide pest and threatens world food production. A previous study indicated that azadirachtin, the most effective botanical insecticide for S. frugiperda, inhibits larval growth of the insect. The effect of azadirachtin on the tissues of the larvae, however, remains to be determined. In this study, the effects of azadirachtin on the structure of fat bodies were analyzed. Comparative transcriptomic analysis was conducted between controls and samples treated with 0.1 μg/g azadirachtin for 7 days to explore potential relevant mechanisms. The expression of 5356 genes was significantly affected after azadirachtin treatment, with 3020 up-regulated and 2336 down-regulated. Among them, 137 encode detoxification enzymes, including 53 P450s, 20 GSTs, 27 CarEs, 16 UGTs, and 12 ABC transporters. Our results indicated that azadirachtin could destroy fat body structure and change the mRNA levels of detoxification-related genes. The up-regulated genes encoding detoxification enzymes might be related to detoxifying azadirachtin. Our results elucidate a preliminary mechanism of azadirachtin detoxification in the fat bodies of S. frugiperda larvae.
秋粘虫，也称草地贪夜蛾，已成为世界性害虫，威胁着世界粮食生产。先前的研究表明，印楝素是防治草地贪夜蛾最有效的植物性杀虫剂，能抑制幼虫的生长。然而，印楝素对幼虫组织的影响仍有待确定。本研究分析了印楝素对脂肪体结构的影响。通过对对照组和用 0.1μg/g 印楝素处理 7 天的样本进行比较转录组分析，以探讨潜在的相关机制。印楝素处理后，有 5356 个基因的表达显著受到影响，其中 3020 个上调，2336 个下调。其中，有 137 个基因编码解毒酶，包括 53 个 P450s、20 个 GSTs、27 个 CarEs、16 个 UGTs 和 12 个 ABC 转运蛋白。研究结果表明，印楝素可以破坏脂肪体结构，并改变与解毒相关的基因的 mRNA 水平。编码解毒酶的上调基因可能与印楝素的解毒有关。本研究阐明了印楝素在草地贪夜蛾幼虫脂肪体中解毒的初步机制。
