School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-sen University, No. 66, Gongchang Rd, Guangming District, Shenzhen, Guangdong 518107, China.
State Key Laboratory of Biocontrol, School of Agriculture and Biotechnology, Sun Yat-sen University, Shenzhen 518107, China.
J Agric Food Chem. 2024 Sep 4;72(35):19323-19332. doi: 10.1021/acs.jafc.4c05290. Epub 2024 Aug 22.
Azadirachtin is a widely used botanical pesticide for agricultural pest control worldwide. However, the molecular mechanisms of azadirachtin in insects are not fully understood. In this study, histological analysis and RNA sequencing were conducted to investigate the impact of azadirachtin on the larval development of . Under azadirachtin exposure, the development was completely inhibited, and the major internal tissues, fat body, and midgut were strongly damaged under histological analysis. Differential gene expression analysis demonstrated that nutrient absorption and detoxification metabolism-related genes are differentially expressed. Interestingly, the expression of the apoptosis-related gene, 8, was significantly inhibited under exposure to azadirachtin. In addition, after knocking down the expression of the 8 gene, the fat body displayed a similar apoptotic phenotype as azadirachtin treatment; the distribution of chromatin and lipid droplets was uneven in the fat body cells. Thus, the results in this study demonstrated that exposure to azadirachtin rapidly activates apoptosis, resulting in innate tissue disruption, ultimately arresting larval development in .
印楝素是一种广泛应用于农业害虫防治的植物性农药，在全球范围内得到广泛应用。然而，印楝素在昆虫中的分子机制尚不完全清楚。本研究通过组织学分析和 RNA 测序，研究了印楝素对 幼虫发育的影响。在印楝素暴露下，幼虫的发育完全被抑制，组织学分析显示主要的内部组织如脂肪体和中肠受到强烈损伤。差异基因表达分析表明，与营养吸收和解毒代谢相关的基因表达存在差异。有趣的是，凋亡相关基因 8 的表达在暴露于印楝素后受到显著抑制。此外，敲低 8 基因的表达后，脂肪体表现出与印楝素处理相似的凋亡表型；脂肪体细胞中的染色质和脂滴分布不均匀。因此，本研究结果表明，暴露于印楝素可迅速激活细胞凋亡，导致固有组织破坏，最终阻止 幼虫的发育。
