Langloss J M, Hoover E A, Kahn D E, Kniazeef A J
Infect Immun. 1978 Jun;20(3):836-41. doi: 10.1128/iai.20.3.836-841.1978.
Feline alveolar macrophages and feline pneumocytes were inoculated in vitro with low multiplicities of either feline calicivirus or feline viral rhinotracheitis virus. Pneumocytes were permissive for both viruses. High titers were attained, and characteristic cytopathic effects developed. Alveolar macrophages were permissive for feline viral rhinotracheitis virus, although the cycle of replication was delayed. Infection of macrophages with feline calicivirus resulted in the production of viral antigens and cytopathic effects; however, viral particles were not detected by electron microscopy, and viral infectivity titers rose only slightly and then fell to undetectable levels by 96 h. The differences in viral susceptibility between these two peripheral pulmonary cell populations that were demonstrated in vitro probably contribute to the differences in pathogenesis of viral rhinotracheitis and calicivirus infections in cats.
用低感染复数的猫杯状病毒或猫病毒性鼻气管炎病毒在体外接种猫肺泡巨噬细胞和猫肺细胞。肺细胞对两种病毒均敏感。病毒滴度很高，并出现了特征性细胞病变效应。肺泡巨噬细胞对猫病毒性鼻气管炎病毒敏感，尽管复制周期有所延迟。猫杯状病毒感染巨噬细胞可导致病毒抗原产生和细胞病变效应；然而，电子显微镜未检测到病毒颗粒，病毒感染性滴度仅略有上升，然后在96小时时降至无法检测的水平。体外实验中这两种肺外周细胞群体对病毒易感性的差异可能导致猫病毒性鼻气管炎和杯状病毒感染发病机制的差异。
