Langloss J M, Hoover E A, Kahn D E, Kniazeff A J
Am J Vet Res. 1979 Feb;40(2):186-9.
In vitro experiments established that the interaction of feline calicivirus (FCV) with alveolar macrophages and pneumocytes results in the generation of chemotactic factors that produce directed migration of neutrophils in Boyden chambers. Factors were produced independent of immune mechanisms and of discernible serum factors. Lysates of noninfected alveolar macrophages and pneumocytes did not possess chemotactic activity, indicating that chemotactic factors were not preformed in these cells. Noninfected alveolar macrophages also elaborated neutrophil chemotactic factors in culture; however, activity was greater when macrophages were infected with FCV. The results of this study suggested that the neutrophilic response in the peripheral portion of the lung of cats exposed to aerosols of FCV was the result of the elaboration of complete chemotactic factors from FCV-infected alveolar cells.
体外实验证实，猫杯状病毒（FCV）与肺泡巨噬细胞和肺细胞相互作用会产生趋化因子，这些趋化因子可使中性粒细胞在博伊登小室中定向迁移。趋化因子的产生与免疫机制和可识别的血清因子无关。未感染的肺泡巨噬细胞和肺细胞裂解物不具有趋化活性，这表明趋化因子并非预先存在于这些细胞中。未感染的肺泡巨噬细胞在培养过程中也会产生中性粒细胞趋化因子；然而，当巨噬细胞感染FCV时，活性会更高。这项研究的结果表明，暴露于FCV气溶胶的猫肺部外周的中性粒细胞反应是FCV感染的肺泡细胞产生完整趋化因子的结果。
