Department of Otorhinolaryngology - Head Neck Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.
Am J Rhinol Allergy. 2022 Jul;36(4):521-528. doi: 10.1177/19458924221086061. Epub 2022 Mar 16.
Neuroimmune communication plays an important role in allergic inflammation, but the neuroimmune regulation of allergic rhinitis remains unclear.
The goal of this study was to investigate the role of CD4-positive T lymphocyte (CD4 T cells) adhesion to D-U87 neuron-like cells in mediating allergic rhinitis CD4 T cell differentiation.
D-U87 neuron-like cells were derived from the human glioblastoma U87 cell line. CD4 T cells were isolated from human peripheral blood using a magnetic separation technique. In vitro coculture of D-U87 neuron-like cells and CD4 T cells was established. The number of adherent CD4 T cells was counted using a fluorescence microscope. The percentages of CD4IFNγ and CD4IL4 T cells and the levels of IFNγ and IL4 cytokines in the supernatant were measured by flow cytometry.
The results showed that the number of adherent CD4 T cells in patients with allergic rhinitis was significantly higher than that in healthy controls. In allergic rhinitis, the percentage of CD4IL4 T cells was significantly increased in the adherent group compared with that in the nonadherent group. Moreover, blocking ICAM1 and E-selectin decreased the number of adherent CD4 T cells and the percentage of CD4IL4 T cells in allergic rhinitis.
Adhesion contributes to CD4IL4 T cell differentiation in the in vitro coculture system of D-U87 neuron-like cells and allergic rhinitis CD4 T cells, which may provide new insights into therapeutic strategies for allergic rhinitis.
神经免疫通讯在变应性炎症中起着重要作用，但变应性鼻炎的神经免疫调节仍不清楚。
本研究旨在探讨 CD4阳性 T 淋巴细胞（CD4 T 细胞）黏附于 D-U87 神经元样细胞在介导变应性鼻炎 CD4 T 细胞分化中的作用。
D-U87 神经元样细胞源自人胶质母细胞瘤 U87 细胞系。采用磁分离技术从人外周血中分离 CD4 T 细胞。建立 D-U87 神经元样细胞与 CD4 T 细胞的体外共培养体系。荧光显微镜下计数黏附的 CD4 T 细胞数。采用流式细胞术检测上清液中 CD4IFNγ和 CD4IL4 T 细胞的百分比以及 IFNγ和 IL4 细胞因子的水平。
结果显示，变应性鼻炎患者黏附的 CD4 T 细胞数量明显高于健康对照组。在变应性鼻炎中，黏附组 CD4IL4 T 细胞的百分比明显高于非黏附组。此外，阻断 ICAM1 和 E-选择素可减少黏附的 CD4 T 细胞数量和变应性鼻炎中 CD4IL4 T 细胞的百分比。
黏附有助于 D-U87 神经元样细胞与变应性鼻炎 CD4 T 细胞体外共培养体系中 CD4IL4 T 细胞的分化，这可能为变应性鼻炎的治疗策略提供新的思路。
