College of Veterinary Medicine, China Agricultural University, Beijing, China.
J Antimicrob Chemother. 2022 May 29;77(6):1557-1560. doi: 10.1093/jac/dkac086.
Recently, epidemiological research has shown an unusually high prevalence of telithromycin-resistant Campylobacter. This study was designed to investigate the potential resistance mechanism of telithromycin resistance in Campylobacter.
A total of 122 Campylobacter isolates of chicken origin collected in 2019 from three regions of China were tested for susceptibility to telithromycin. The potential mechanism of resistance to telithromycin in Campylobacter was revealed through WGS analysis and natural transformation.
In this study, 51.3% (61/119) of Campylobacter coli and 100.0% (3/3) of Campylobacter jejuni were resistant to telithromycin. erm(B) or A2075G mutation in 23S rRNA (23S_A2075G) was identified in the telithromycin-resistant C. coli. Cloning of the erm(B) or 23S_A2075G into C. jejuni NCTC 11168 resulted in a 256-fold increase in the MIC of telithromycin. MLST results indicated that various STs were involved in the dissemination of 23S_A2075G and erm(B). Phylogenetic analysis showed that the C. coli isolates with 23S_A2075G and erm(B) from chickens and humans were closely related.
23S_A2075G and erm(B), which have been widely spread in different genotypes of C. coli isolated from animals and humans, could mediate high levels of resistance to telithromycin in C. coli. C. coli containing 23S_A2075G or erm(B) are clonally related and have the potential to spread zoonotic diseases.
最近，流行病学研究表明，弯曲菌对克拉霉素的耐药率异常高。本研究旨在探讨弯曲菌对克拉霉素耐药的潜在耐药机制。
本研究共检测了 2019 年从中国三个地区采集的 122 株鸡源弯曲菌对克拉霉素的敏感性。通过 WGS 分析和自然转化揭示了弯曲菌对克拉霉素耐药的潜在机制。
本研究中，51.3%（61/119）的大肠杆菌和 100.0%（3/3）的空肠弯曲菌对克拉霉素耐药。在耐药的大肠杆菌中发现了 23S rRNA（23S_A2075G）中的 erm(B)或 A2075G 突变。将 erm(B)或 23S_A2075G 克隆到空肠弯曲菌 NCTC 11168 中，导致克拉霉素的 MIC 增加了 256 倍。MLST 结果表明，不同的 ST 参与了 23S_A2075G 和 erm(B)的传播。系统发育分析表明，来自鸡和人类的带有 23S_A2075G 和 erm(B)的大肠杆菌分离株密切相关。
在动物和人类分离的不同基因型的大肠杆菌中广泛传播的 23S_A2075G 和 erm(B)可介导对克拉霉素的高水平耐药。含有 23S_A2075G 或 erm(B)的大肠杆菌具有克隆相关，并有传播人畜共患病的潜力。
