柠檬酸铁铵分解——一种用于革兰氏阴性菌的分类工具。

Ferric ammonium citrate decomposition--a taxonomic tool for gram-negative bacteria.

作者信息

Szentmihályi A, Lányi B

出版信息

Acta Microbiol Hung. 1986;33(1):85-95.

Abstract

The iron uptake test of Szabó and Vandra has been modified and used for the differentiation of Gram-negative bacteria. Nutrient agar containing 20 g per litre of ferric ammonium citrate was distributed into narrow tubes and solidified so as to form butts and slants. Considering the localization of the rusty-brown coloration produced after seeding and incubation, 2367 strains were classified into four groups. (1) Unchanged medium: Escherichia coli, Shigella spp., Yersinia spp., Hafnia alvei and Morganella morganii 100% each, Klebsiella spp., 50%, Enterobacter cloacae 37%, Proteus vulgaris 59%, Acinetobacter spp. 42%, Pseudomonas fluorescens 19%, some other bacteria 2-12%. (2) Rusty-brown slant, unchanged butt: Salmonella subgenera II, III and IV 98%, Citrobacter freundii 65%, E. cloacae 55%, P. vulgaris 41%, Proteus mirabilis 98%, Providencia rettgeri 100%, urease-negative Providencia 96%, Acinetobacter spp. 58%, Pseudomonas aeruginosa 100%, P. fluorescens 81%, UFP (unclassified fluorescent pseudomonads) 100%, other Pseudomonas spp. 55%. (3) Unchanged slant, brown butt: S. typhi 88%, Salmonella subgenus I 3%, Klebsiella spp. 31%, some other bacteria 2-3%. (4) Rusty-brown slant, brown butt: Salmonella subgenus I 75%, C. freundii 20%, Klebsiella spp. 12%, some other bacteria 1-5%. Colour reactions in ferric ammonium citrate agar are associated with the accumulation of ferric hydroxide: bacteria giving positive reactions on the slant took up as an average, 63 times more iron than those with negative test. The localization of colour reaction correlated partly with aerobic and anaerobic citrate utilization or decomposition in Simmons' minimal and in Kauffmann's peptone water medium.

摘要

萨博（Szabó）和万德拉（Vandra）的铁摄取试验已被改良，并用于革兰氏阴性菌的鉴别。将每升含20克柠檬酸铁铵的营养琼脂分装到细管中并凝固，以形成底部和斜面。根据接种和培养后产生的锈褐色着色的位置，将2367株菌株分为四组。（1）培养基不变：大肠杆菌、志贺氏菌属、耶尔森氏菌属、蜂房哈夫尼亚菌和摩根氏摩根菌各100%，克雷伯氏菌属50%，阴沟肠杆菌37%，普通变形杆菌59%，不动杆菌属42%，荧光假单胞菌19%，其他一些细菌2% - 12%。（2）斜面呈锈褐色，底部不变：沙门氏菌属II、III和IV组98%，弗氏柠檬酸杆菌65%，阴沟肠杆菌55%，普通变形杆菌41%，奇异变形杆菌98%，雷氏普罗威登斯菌100%，脲酶阴性的普罗威登斯菌96%，不动杆菌属58%，铜绿假单胞菌100%，荧光假单胞菌81%，未分类荧光假单胞菌（UFP）100%，其他假单胞菌属55%。（3）斜面不变，底部呈褐色：伤寒沙门氏菌88%，沙门氏菌属I组3%，克雷伯氏菌属31%，其他一些细菌2% - 3%。（4）斜面呈锈褐色，底部呈褐色：沙门氏菌属I组75%，弗氏柠檬酸杆菌20%，克雷伯氏菌属12%，其他一些细菌1% - 5%。柠檬酸铁铵琼脂中的颜色反应与氢氧化铁的积累有关：在斜面上产生阳性反应的细菌平均摄取的铁比试验阴性的细菌多63倍。颜色反应的位置部分与在西蒙斯（Simmons）基本培养基和考夫曼（Kauffmann）蛋白胨水培养基中需氧和厌氧利用或分解柠檬酸盐有关。